Characterization of paromomycin sulfate by capillary electrophoresis with UV detection after pre-capillary derivatization

A capillary electrophoretic method has been developed for determination of the components of the paromomycin mixture salt. Paromomycin was detected at 330 rim after pre-capillary derivatization with o-phthaldialdehyde and thioglycolic acid. The electrophoretic separation was performed in a fused-silica capillary at 25degreesC and 18 kV with a background electrolyte comprising 40 mm sodium tetraborate, 3 mm beta-cyclodextrin, and 12.5% (v/v) methanol. Although the analysis time was reduced to 10 min, five peaks could be separated to baseline. The relative standard deviation of the ratios (peak area/internal standard peak area) was <5% for all peaks.