ATP is a mediator of the fast inhibitory junction potential in human jejunal circular smooth muscle

被引:60
作者
Xue, L
Farrugia, G
Sarr, MG
Szurszewski, JH
机构
[1] Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA
[2] Mayo Clin & Mayo Fdn, Div Gastroenterol & Hepatol, Rochester, MN 55905 USA
[3] Mayo Clin & Mayo Fdn, Dept Surg, Rochester, MN 55905 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 1999年 / 276卷 / 06期
关键词
neurotransmission; microelectrodes; purinergic receptors;
D O I
10.1152/ajpgi.1999.276.6.G1373
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The neurotransmitter(s) that generates the fast component of the inhibitory junction potential (IJP-F) in human jejunal circular smooth muscle is not known. The aim of this study was to determine the role of ATP and purinergic receptors in the generation of the IJP-F in human jejunal circular smooth muscle strips. The P-2-receptor antagonist suramin (100 mu M) reduced the IJP-F by 28%. Apamin (1 mu M) reduced the IJP-F by 25%. Desensitization of muscle strips with the putative P-2x-receptor agonist alpha,beta-methylene ATP (alpha,beta-MeATP, 100 mu M) decreased the IJP-F by 44%, and desensitization with the putative P-2y-receptor agonist adenosine 5'-O-2-thiodiphosphate (ADP beta S) completely abolished the IJP-F. Desensitization with the putative P-2y-receptor agonist 2-methylthioATP had no effect on the IJP-F. Exogenous ATP evoked a hyperpolarization with a time course that matched the IJP-F. The ATP-evoked hyperpolarization was reduced by apamin and suramin, reduced by desensitization with alpha,beta-MeATP (69% decrease), and abolished by desensitization with ADP beta S. These data suggest that the IJP-F in human jejunal circular smooth muscle is mediated in part by ATP through an ADP beta S-sensitive P-2 receptor.
引用
收藏
页码:G1373 / G1379
页数:7
相关论文
共 25 条
[11]   NERVOUS RELEASE AND ACTION OF SUBSTANCES WHICH AFFECT INTESTINAL MUSCLE THROUGH NEITHER ADRENORECEPTORS NOR CHOLINORECEPTORS [J].
FURNESS, JB ;
COSTA, M .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 1973, 265 (867) :123-133
[12]   NITRIC-OXIDE INVOLVEMENT IN THE PEPTIDE VIP-ASSOCIATED INHIBITORY JUNCTION POTENTIAL IN THE GUINEA-PIG ILEUM [J].
HE, XD ;
GOYAL, RK .
JOURNAL OF PHYSIOLOGY-LONDON, 1993, 461 :485-499
[13]   Small-conductance Ca2+-dependent K+ channels activated by ATP in murine colonic smooth muscle [J].
Koh, SD ;
Dick, GM ;
Sanders, KM .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1997, 273 (06) :C2010-C2021
[14]   SOME CHARACTERISTICS OF TRANSMISSION FROM NON-ADRENERGIC, NON-CHOLINERGIC EXCITATORY NERVES TO THE SMOOTH-MUSCLE OF THE CHICKEN [J].
KOMORI, S ;
OHASHI, H .
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1982, 6 (02) :199-210
[15]  
MAGGI CA, 1993, N-S ARCH PHARMACOL, V347, P630
[16]   EVIDENCE THAT GABAA RECEPTORS MEDIATE RELAXATION OF RAT DUODENUM BY ACTIVATING INTRAMURAL NONADRENERGIC-NON-CHOLINERGIC NEURONS [J].
MAGGI, CA ;
MANZINI, S ;
MELI, A .
JOURNAL OF AUTONOMIC PHARMACOLOGY, 1984, 4 (02) :77-85
[17]   EFFECT OF SUBSTANCE-P ON NON-CHOLINERGIC FAST AND SLOW POST-STIMULUS DEPOLARIZATION IN THE GUINEA-PIG ILEUM [J].
NIEL, JP ;
BYWATER, RAR ;
TAYLOR, GS .
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1983, 9 (04) :573-584
[18]   APAMIN-RESISTANT POST-STIMULUS HYPERPOLARIZATION IN THE CIRCULAR MUSCLE OF THE GUINEA-PIG ILEUM [J].
NIEL, JP ;
BYWATER, RAR ;
TAYLOR, GS .
JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 1983, 9 (2-3) :565-569
[19]   EFFECT OF NITRIC-OXIDE ON CIRCULAR MUSCLE OF THE CANINE SMALL-INTESTINE [J].
STARK, ME ;
BAUER, AJ ;
SZURSZEWSKI, JH .
JOURNAL OF PHYSIOLOGY-LONDON, 1991, 444 :743-761
[20]   NITRIC-OXIDE MEDIATES INHIBITORY NERVE INPUT IN HUMAN AND CANINE JEJUNUM [J].
STARK, ME ;
BAUER, AJ ;
SARR, MG ;
SZURSZEWSKI, JH .
GASTROENTEROLOGY, 1993, 104 (02) :398-409