Coxiella burnetii DNA, But Not Viable Bacteria, in Dairy Products in France

被引：69

作者：

Eldin, Carole ^{[1
]}

Angelakis, Emmanouil ^{[1
]}

Renvoise, Aurelie ^{[1
]}

Raoult, Didier ^{[1
]}

机构：

[1] Univ Mediterranee, Fac Med, URMITE, F-13385 Marseille 5, France

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2013年 / 88卷 / 04期

关键词：

Q-FEVER; RAW-MILK; PREVALENCE; GOATS; COWS;

D O I：

10.4269/ajtmh.12-0212

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Transmission by the oral route of Coxiella burnetii is controversial. Our objective was to evaluate dairy products in the transmission of Q fever. Pasteurized, unpasteurized, and thermized dairy products were tested for C. burnetii by using a quantitative polymerase chain reaction specific for IS1111 and IS30A spacers, culturing in human embryonic lung fibroblasts cells, and inoculation into BALB/c mice. We tested 201 products and C. burnetii was identified in 64%. Cow milk origin products were more frequently positive than goat or ewe products (P = 0.006 and P = 0.0001, respectively), and industrial food was more frequently positive than artisanal food (P < 0.0001). Food made from unpasteurized milk contained higher bacteria concentrations than food made from pasteurized milk (P = 0.02). All cultures were negative and mice did not show signs of illness. Farm animals are highly infected in France but consumption of cheese and yogurt does not seem to pose a public health risk for transmission of Q fever.

引用

页码：765 / 769

页数：5

共 32 条

[1] Q fever [J].

Angelakis, Emmanouil ;

Raoult, Didier .

VETERINARY MICROBIOLOGY, 2010, 140 (3-4) :297-309

[2] Kinetics of Coxiella burnetii excretion in a commercial dairy sheep flock after treatment with oxytetracycline [J].

Astobiza, Ianire ;

Barandika, Jesus F. ;

Hurtado, Ana ;

Juste, Ramon A. ;

Garcia-Perez, Ana L. .

VETERINARY JOURNAL, 2010, 184 (02) :172-175

[3] Q-FEVER STUDIES IN SOUTHERN CALIFORNIA .2. AN EPIDEMIOLOGICAL STUDY OF 300 CASES [J].

BECK, MD ;

BELL, JA ;

SHAW, EW ;

HUEBNER, RJ .

PUBLIC HEALTH REPORTS, 1949, 64 (02) :41-62

[4] SEROLOGIC ANALYSIS OF A PENITENTIARY GROUP USING RAW-MILK FROM A Q-FEVER INFECTED HERD [J].

BENSON, WW ;

BROCK, DW ;

MATHER, J .

PUBLIC HEALTH REPORTS, 1963, 78 (08) :707-710

[5]

BIBERSTE.EL, 1974, AM J VET RES, V35, P1577

[6] Coxiella burnetii and milk pasteurization:: an early application of the precautionary principle? [J].

Cerf, O. ;

Condron, R. .

EPIDEMIOLOGY AND INFECTION, 2006, 134 (05) :946-951

[7] Detection of Coxiella burnetii DNA on Small-Ruminant Farms during a Q Fever Outbreak in the Netherlands [J].

de Bruin, A. ;

van der Plaats, R. Q. J. ;

de Heer, L. ;

Paauwe, R. ;

Schimmer, B. ;

Vellema, P. ;

van Rotterdam, B. J. ;

van Duynhoven, Y. T. H. P. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (06) :1652-1657

[8] A CLUSTER OF COXIELLA-BURNETII INFECTIONS ASSOCIATED WITH EXPOSURE TO VACCINATED GOATS AND THEIR UNPASTEURIZED DAIRY-PRODUCTS [J].

FISHBEIN, DB ;

RAOULT, D .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1992, 47 (01) :35-40

[9] Diagnosis of Q fever [J].

Fournier, PE ;

Marrie, TJ ;

Raoult, D .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :1823-1834

[10] Q Fever in France, 1985-2009 [J].

Frankel, Diane ;

Richet, Herve ;

Renvoise, Aurelie ;

Raoult, Didier .

EMERGING INFECTIOUS DISEASES, 2011, 17 (03) :350-356

← 1 2 3 4 →