Comparison of separation modes of high-performance liquid chromatography for the analysis of glycoprotein- and proteoglycan-derived oligosaccharides

被引：29

作者：

Davies, MJ ^{[1
]}

Hounsell, EF ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOLEC BIOL,GLYCOPROT STRUCT FUNCT GRP,LONDON WC1E 6BT,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 720卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0021-9673(95)00221-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Porous graphitised carbon (PGC) has been explored for high-performance liquid chromatography (HPLC) of mono- and di-saccharides released from proteoglycans and of fluorescently labelled oligosaccharide derivatives for high-sensitivity detection. Sulphated oligosaccharides show good retention and separation behaviour on PGC-HPLC, and compared to anion-exchange or reversed-phase ion-pair chromatography the chromatography is carried out in the absence of salt. Due to their poor retention on PGC-HPLC the analysis of single uronic acids has been optimised with high pH anion-exchange chromatography. Fluorescent labelled derivatives formed by reductive amination of neutral oligosaccharides with 2-aminobenzamide have been chromatographed on PGC-HPLC and by BioGel P4 gel filtration.

引用

收藏

页码：227 / 233

页数：7

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