A thermostable D-hydantoinase isolated from a Mesophilic bacillus sp AR9

被引：16

作者：

Sharma, R ^{[1
]}

Vohra, RM ^{[1
]}

机构：

[1] INST MICROBIAL TECHNOL, BIOCHEM ENGN RES & PROC DEV CTR, CHANDIGARH 160036, INDIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 234卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.6659

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A thermostable hydantoinase has been characterized from a mesophilic Bacillus sp.AR9. The hydantoinase produced by this Bacillus sp.AR9 is strictly D-specific and is constitutively produced with high yields (4500 U/ml) in this strain. The enzyme is not only alkalo- and thermostable but has a pH and temperature optimum of 9.5 and 65 degrees C, respectively, which is advantageous for the bioconversion of DL-5-monosubstituted-hydantoin derivatives, The enzyme has a half life of 80 minutes at 70 degrees C and loses only 33% of its activity in 4 hr at 60 degrees C, The enzyme has a broad substrate specificity with a maximum of 100% with hydantoin and about 26% with dihydrouracil. Co++ ions enhance the activity of the enzyme by more than 60%. (C) 1997 Academic Press.

引用

页码：485 / 488

页数：4

共 16 条

[11] PROPERTIES OF THE HYDANTOINASE FROM AGROBACTERIUM SP IP-I-671 [J].

RUNSER, S ;

OHLEYER, E .

BIOTECHNOLOGY LETTERS, 1990, 12 (04) :259-264

[12] OPTICAL-ROTATORY DISPERSION AND CIRCULAR-DICHROISM OF AMINO-ACID HYDANTOINS [J].

SUZUKI, T ;

IGARASHI, K ;

HASE, K ;

TUZIMURA, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1973, 37 (02) :411-416

[13]

TAKAHASHI S, 1978, J FERMENT TECHNOL, V56, P492

[14]

TAKAHASHI S, 1979, J FERMENT TECHNOL, V57, P328

[15]

YAMADA H, 1978, J FERMENT TECHNOL, V56, P484

[16] ENZYMATIC PRODUCTION OF D-AMINO ACIDS FROM 5-SUBSTITUTED HYDANTOINS .1. SCREENING OF MICROORGANISMS PRODUCING D-P-HYDROXYPHENYL-GLYCINE FROM DL-5-(P-HYDROXYPHENYL)HYDANTOIN [J].

YOKOZEKI, K ;

NAKAMORI, S ;

EGUCHI, C ;

YAMADA, K ;

MITSUGI, K .

AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1987, 51 (02) :355-362

← 1 2 →