Lipoxygenase6-Dependent Oxylipin Synthesis in Roots Is Required for Abiotic and Biotic Stress Resistance of Arabidopsis

被引:117
作者
Grebner, Wiebke [1 ]
Stingl, Nadja E. [1 ]
Oenel, Ayla [1 ]
Mueller, Martin J. [1 ]
Berger, Susanne [1 ]
机构
[1] Univ Wurzburg, Bioctr, Julius von Sachs Inst Biosci, Dept Pharmaceut Biol, D-97082 Wurzburg, Germany
关键词
JASMONIC ACID; DEFENSE RESPONSES; METHYL JASMONATE; GENE-EXPRESSION; MALE-STERILE; ACCUMULATION; BIOSYNTHESIS; BIOGENESIS; SENESCENCE; MUTANTS;
D O I
10.1104/pp.113.214544
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Jasmonates are oxylipin signals that play important roles in the development of fertile flowers and in defense against pathogens and herbivores in leaves. The aim of this work was to understand the synthesis and function of jasmonates in roots. Grafting experiments with a jasmonate-deficient mutant demonstrated that roots produce jasmonates independently of leaves, despite low expression of biosynthetic enzymes. Levels of 12-oxo-phytodienoic acid, jasmonic acid, and its isoleucine derivative increased in roots upon osmotic and drought stress. Wounding resulted in a decrease of preformed 12-oxo-phytodienoic acid concomitant with an increase of jasmonic acid and jasmonoyl-isoleucine. 13-Lipoxygenases catalyze the first step of lipid oxidation leading to jasmonate production. Analysis of 13-lipoxygenase-deficient mutant lines showed that only one of the four 13-lipoxygenases, LOX6, is responsible and essential for stress-induced jasmonate accumulation in roots. In addition, LOX6 was required for production of basal 12-oxo-phytodienoic acid in leaves and roots. Loss-of-function mutants of LOX6 were more attractive to a detritivorous crustacean and more sensitive to drought, indicating that LOX6-derived oxylipins are important for the responses to abiotic and biotic factors.
引用
收藏
页码:2159 / 2170
页数:12
相关论文
共 49 条
[1]   The immediate activation of defense responses in Arabidopsis roots is not sufficient to prevent Phytophthora parasitica infection [J].
Attard, Agnes ;
Gourgues, Mathieu ;
Callemeyn-Torre, Nicolas ;
Keller, Harald .
NEW PHYTOLOGIST, 2010, 187 (02) :449-460
[2]   Diversity of the Enzymatic Activity in the Lipoxygenase Gene Family of Arabidopsis thaliana [J].
Bannenberg, Gerard ;
Martinez, Marta ;
Hamberg, Mats ;
Castresana, Carmen .
LIPIDS, 2009, 44 (02) :85-95
[3]   CHARACTERIZATION OF AN ARABIDOPSIS-LIPOXYGENASE GENE RESPONSIVE TO METHYL JASMONATE AND WOUNDING [J].
BELL, E ;
MULLET, JE .
PLANT PHYSIOLOGY, 1993, 103 (04) :1133-1137
[4]   A CHLOROPLAST LIPOXYGENASE IS REQUIRED FOR WOUND-INDUCED JASMONIC ACID ACCUMULATION IN ARABIDOPSIS [J].
BELL, E ;
CREELMAN, RA ;
MULLET, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (19) :8675-8679
[5]   The fou2 mutation in the major vacuolar cation channel TPC1 confers tolerance to inhibitory luminal calcium [J].
Beyhl, Diana ;
Hoertensteiner, Stefan ;
Martinoia, Enrico ;
Farmer, Edward E. ;
Fromm, Joerg ;
Marten, Irene ;
Hedrich, Rainer .
PLANT JOURNAL, 2009, 58 (05) :715-723
[6]   A gain-of-function allele of TPC1 activates oxylipin biogenesis after leaf wounding in Arabidopsis [J].
Bonaventure, Gustavo ;
Gfeller, Aurelie ;
Proebsting, William M. ;
Hoertensteiner, Stefan ;
Chetelat, Aurore ;
Martinoia, Enrico ;
Farmer, Edward E. .
PLANT JOURNAL, 2007, 49 (05) :889-898
[7]   Crystal structure of 12-oxophytodienoate reductase 3 from tomato: Self-inhibition by dimerization [J].
Breithaupt, Constanze ;
Kurzbauer, Robert ;
Lilie, Hauke ;
Schaller, Andreas ;
Strassner, Jochen ;
Huber, Robert ;
Macheroux, Peter ;
Clausen, Tim .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (39) :14337-14342
[8]   New weapons and a rapid response against insect attack [J].
Browse, John ;
Howe, Gregg A. .
PLANT PHYSIOLOGY, 2008, 146 (03) :832-838
[9]   The power of mutants for investigating jasmonate biosynthesis and signaling [J].
Browse, John .
PHYTOCHEMISTRY, 2009, 70 (13-14) :1539-1546
[10]   Jasmonate Passes Muster: A Receptor and Targets for the Defense Hormone [J].
Browse, John .
ANNUAL REVIEW OF PLANT BIOLOGY, 2009, 60 :183-205