PfSUB-2:: a second subtilisin-like protein in Plasmodium falciparum merozoites

被引：56

作者：

Hackett, F ^{[1
]}

Sajid, M ^{[1
]}

Withers-Martinez, C ^{[1
]}

Grainger, M ^{[1
]}

Blackman, MJ ^{[1
]}

机构：

[1] Natl Inst Med Res, Div Parasitol, London NW7 1AA, England

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1999年 / 103卷 / 02期

基金：

英国医学研究理事会;

关键词：

malaria; merozoite; subtilisin; protease; post-translational processing;

D O I：

10.1016/S0166-6851(99)00122-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Erythrocyte invasion by the malaria merozoite requires the activity of merozoite proteases. We have previously identified a Plasmodium falciparum protein belonging to the superfamily of subtilisin-like serine proteases, which is expressed in a subset of secretory organelles in free merozoites. Here we describe the identification of a second P. falciparum subtilisin-like merozoite protein. Called PfSUB-2, it is encoded by a single copy gene and is expressed as a large putative type I integral membrane protein which undergoes extensive post-translational processing. The terminal processing product is expressed in an apical location in merozoites. PfSUB-2 may mediate one or more of the serine protease activities known to be associated with erythrocyte invasion. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：183 / 195

页数：13

共 35 条

[1] Activation of the furin endoprotease is a multiple-step process: Requirements for acidification and internal propeptide cleavage [J].

Anderson, ED ;

VanSlyke, JK ;

Thulin, CD ;

Jean, F ;

Thomas, G .

EMBO JOURNAL, 1997, 16 (07) :1508-1518

[2] INVOLVEMENT OF MALARIAL PROTEASES IN THE INTERACTION BETWEEN THE PARASITE AND HOST ERYTHROCYTE IN PLASMODIUM-KNOWLESI INFECTIONS [J].

BANYAL, HS ;

MISRA, GC ;

GUPTA, CM ;

DUTTA, GP .

JOURNAL OF PARASITOLOGY, 1981, 67 (05) :623-626

[3] Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite surface protein 1-42 maturase [J].

Barale, JC ;

Blisnick, T ;

Fujioka, H ;

Alzari, PM ;

Aikawa, M ;

Braun-Breton, C ;

Langsley, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (11) :6445-6450

[4] A subtilisin-like protein in secretory organelles of Plasmodium falciparum merozoites [J].

Blackman, MJ ;

Fujioka, H ;

Stafford, WHL ;

Sajid, M ;

Clough, B ;

Fleck, SL ;

Aikawa, M ;

Grainger, M ;

Hackett, F .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (36) :23398-23409

[5] A CONSERVED PARASITE SERINE-PROTEASE PROCESSES THE PLASMODIUM-FALCIPARUM MEROZOITE SURFACE PROTEIN-1 [J].

BLACKMAN, MJ ;

CHAPPEL, JA ;

SHAI, S ;

HOLDER, AA .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 62 (01) :103-114

[6]

BLACKMAN MJ, 1994, METHOD CELL BIOL, V45, P213

[7] PROCESSING OF THE PLASMODIUM-FALCIPARUM MAJOR MEROZOITE SURFACE PROTEIN-1 - IDENTIFICATION OF A 33-KILODALTON SECONDARY PROCESSING PRODUCT WHICH IS SHED PRIOR TO ERYTHROCYTE INVASION [J].

BLACKMAN, MJ ;

WHITTLE, H ;

HOLDER, AA .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 49 (01) :35-44

[8] A SINGLE FRAGMENT OF A MALARIA MEROZOITE SURFACE PROTEIN REMAINS ON THE PARASITE DURING RED-CELL INVASION AND IS THE TARGET OF INVASION-INHIBITING ANTIBODIES [J].

BLACKMAN, MJ ;

HEIDRICH, HG ;

DONACHIE, S ;

MCBRIDE, JS ;

HOLDER, AA .

JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (01) :379-382

[9] Plasmodium knowlesi secondary processing of the malaria merozoite surface protein-1 [J].

Blackman, MJ ;

Dennis, ED ;

Hirst, EMA ;

Kocken, CH ;

ScottFinnigan, TJ ;

Thomas, AW .

EXPERIMENTAL PARASITOLOGY, 1996, 83 (02) :229-239

[10] INDUCTION OF THE PROTEOLYTIC ACTIVITY OF A MEMBRANE-PROTEIN IN PLASMODIUM-FALCIPARUM BY PHOSPHATIDYL INOSITOL-SPECIFIC PHOSPHOLIPASE-C [J].

BRAUNBRETON, C ;

ROSENBERRY, TL ;

DASILVA, LP .

NATURE, 1988, 332 (6163) :457-459

← 1 2 3 4 →