Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy

被引:560
作者
Lekka, M
Laidler, P
Gil, D
Lekki, J
Stachura, Z
Hrynkiewicz, AZ
机构
[1] Inst Nucl Phys, PL-31342 Krakow, Poland
[2] Inst Med Biochem Collegium Medicum, PL-31034 Krakow, Poland
来源
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 1999年 / 28卷 / 04期
关键词
scanning force microscopy; cytoskeleton elasticity; normal and cancerous cells;
D O I
10.1007/s002490050213
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Scanning force microscopy was used for the determination of the elastic properties of living cells in their culture conditions. The studies were carried out an human epithelial cells. Two similar lines of normal cells (Hu609 and HCV29) and three cancerous ones (Hu456, T24, BC3726) were measured using the scanning force microscope in order to collect the force versus indentation curves. The BC3726 line originates from the HCV29 cell line which was transformed by the v-ras oncogene. To evaluate their elastic properties, Young's modulus values were determined. The present study has shown that normal cells have a Young's modulus of about one order of magnitude higher than cancerous ones. Such a change might be attributed to a difference in the organisation of cell cytoskeletons and requires further studies.
引用
收藏
页码:312 / 316
页数:5
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