Probing cysteine reactivity in proteins by mass spectrometric EC-tagging

被引：37

作者：

Dayon, L ^{[1
]}

Roussel, C ^{[1
]}

Girault, HH ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Lab Electrochim Phys & Analyt, CH-1015 Lausanne, Switzerland

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 04期

关键词：

cysteine markers; electrochemistry; electrospray; mass spectrometry; protein structures probing;

D O I：

10.1021/pr050365o

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The on-line electrochemical tagging (EC-tagging) of cysteine residues in proteins during mass spectrometry is studied to probe the cysteine environment. Benzoquinone probes electrogenerated at a microspray electrode react with the thiol functions of the proteins within a microchannel and the products are analyzed by mass spectrometry. The fundamentals of the technique are discussed, with a focus on the kinetic aspects. The EC-tagging efficiency of the cysteine residues in proteins is used to probe their environment. Experiments with unmodified proteins and their chemically reduced forms highlight the strong effect of the cysteine site reactivity on the tagging efficiencies. This study highlights relevant parameters for such on-line electrochemical derivatization/MS detection strategies.

引用

页码：793 / 800

页数：8

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