Differentially promoted peripheral nerve regeneration by grafted Schwann cells over-expressing different FGF-2 isoforms

被引：105

作者：

Haastert, K

Lipokatic, E

Fischer, M

Timmer, M

Grothe, C

机构：

[1] Hannover Med Sch, Dept Neuroanat, D-30625 Hannover, Germany

[2] Ctr Syst Neurosci, Hannover, Germany

[3] Hannover Med Sch, Dept Neurophysiol, D-30625 Hannover, Germany

来源：

NEUROBIOLOGY OF DISEASE | 2006年 / 21卷 / 01期

关键词：

fibroblast growth factor-2; isoforms; peripheral nerve regeneration; cell therapy; neurotrophic therapy; Schwann cells; genetic modification;

D O I：

10.1016/j.nbd.2005.06.020

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Artificial nerve grafts are needed to reconstruct massive defects in the peripheral nervous system when autologous nerve grafts are not available in sufficient amounts. Nerve grafts containing Schwann cells display a suitable substrate for long-distance regeneration. We present here a comprehensive analysis of the in vivo effects of different isoforms of fibroblast growth factor-2 (FGF-2) on peripheral nerve regeneration across long gaps. FGF-2 isoforms were provided by grafted, genetically modified Schwann cells over-expressing 18-kDa-FGF-2 and 21-/23-kDa-FGF-2, respectively. Functional tests evaluated motor and sensory recovery. Additionally, morphometrical analyses of regenerated nerves were performed 3 and 6 months after grafting. Distinct regeneration promoting effects of the different FGF-2 isoforms were found. 18-kDa-FGF-2 mediated inhibitory effects on the grade of myelination of regenerating axons, whereas 21-/23-kDa-FGF-2 mediated early recovery of sensory functions and stimulation of long-distance myelination of regenerating axons. The results contribute to the development of new therapeutic strategies in peripheral nerve repair. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：138 / 153

页数：16

共 51 条

[1] BASIC FIBROBLAST GROWTH-FACTOR RELEASED FROM SYNTHETIC GUIDANCE CHANNELS FACILITATES PERIPHERAL-NERVE REGENERATION ACROSS LONG NERVE GAPS [J].