Acellular muscle with Schwann-cell implantation:: An alternative biologic nerve conduit

被引:37
作者
Fansa, H [1 ]
Keilhoff, G [1 ]
Förster, G [1 ]
Seidel, B [1 ]
Wolf, G [1 ]
Schneider, W [1 ]
机构
[1] Univ Magdeburg, Dept Plast Reconstruct & Hand Surg, D-39106 Magdeburg, Germany
关键词
D O I
10.1055/s-2007-1000134
中图分类号
R61 [外科手术学];
学科分类号
摘要
Denatured or acellular muscle grafts are known to support axonal regeneration. With increasing gap length, failure of regeneration is evident, due to the lack of viable Schwann cells in the graft. The authors created a biologic nerve conduit, in a rat sciatic nerve model, by implanting cultured Schwann cells into an acellular gracilis muscle. Autologous nerve grafts and acellular muscle grafts without Schwann cells served as controls. After 6 weeks, regeneration was assessed clinically, histologically, and morphometrically. Polymerase chain reaction (PCR) analysis showed that the implanted Schwann cells remained viable within the graft. Good regeneration was noted in the muscle-Schwann cell group, while regeneration in the muscle grafts without Schwann cells was significantly impaired. The muscle-Schwann cell graft demonstrated systematic and organized regeneration, including the proper orientation of regenerated fibers. The number of axons regenerating through the muscle-Schwann cell grafts was significantly increased, compared with the acellular muscle without Schwann cells. Implantation of Schwann cells into acellular muscle thus provided a biologic conduit with large basal lamina tubes, as a pathway for regenerating axons. The positive effects of Schwann cells, producing neurotrophic and neurotropic factors, supported axonal regeneration.
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页码:531 / 537
页数:7
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共 36 条
  • [1] Peripheral nerve regeneration through nerve guides seeded with adult Schwann cells
    Ansselin, AD
    Fink, T
    Davey, DF
    [J]. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY, 1997, 23 (05) : 387 - 398
  • [2] Bunge R P, 1993, Curr Opin Neurobiol, V3, P805, DOI 10.1016/0959-4388(93)90157-T
  • [3] NERVE-MUSCLE SANDWICH GRAFTS - THE IMPORTANCE OF SCHWANN-CELLS IN PERIPHERAL-NERVE REGENERATION THROUGH MUSCLE BASAL LAMINA CONDUITS
    CALDER, JS
    GREEN, CJ
    [J]. JOURNAL OF HAND SURGERY-BRITISH AND EUROPEAN VOLUME, 1995, 20B (04) : 423 - 428
  • [4] DENATURED MUSCLE AS A NERVE CONDUIT - A FUNCTIONAL, MORPHOLOGIC, AND ELECTROPHYSIOLOGIC EVALUATION
    CHEN, LE
    SEABER, AV
    URBANIAK, JR
    MURRELL, GAC
    [J]. JOURNAL OF RECONSTRUCTIVE MICROSURGERY, 1994, 10 (03) : 137 - 144
  • [5] PREDEGENERATION ENHANCES REGENERATION INTO ACELLULAR NERVE GRAFTS
    DANIELSEN, N
    KERNS, JM
    HOLMQUIST, B
    ZHAO, Q
    LUNDBORG, G
    KANJE, M
    [J]. BRAIN RESEARCH, 1995, 681 (1-2) : 105 - 108
  • [6] Successful implantation of Schwann cells in acellular muscles
    Fansa, H
    Keilhoff, G
    Plogmeier, K
    Frerichs, O
    Wolf, G
    Schneider, W
    [J]. JOURNAL OF RECONSTRUCTIVE MICROSURGERY, 1999, 15 (01) : 61 - 65
  • [7] MUSCLE BASAL LAMINA - A NEW GRAFT MATERIAL FOR PERIPHERAL-NERVE REPAIR
    FAWCETT, JW
    KEYNES, RJ
    [J]. JOURNAL OF NEUROSURGERY, 1986, 65 (03) : 354 - 363
  • [8] THE ROLE OF SCHWANN-CELLS IN THE REGENERATION OF PERIPHERAL-NERVE AXONS THROUGH MUSCLE BASAL LAMINA GRAFTS
    FENELEY, MR
    FAWCETT, JW
    KEYNES, RJ
    [J]. EXPERIMENTAL NEUROLOGY, 1991, 114 (03) : 275 - 285
  • [9] Sciatic nerve regeneration through venous or nervous grafts in the rat
    FoidartDessalle, M
    Dubuisson, A
    Lejeune, A
    Severyns, A
    Manassis, Y
    Delree, P
    Crielaard, JM
    Bassleer, R
    Lejeune, G
    [J]. EXPERIMENTAL NEUROLOGY, 1997, 148 (01) : 236 - 246
  • [10] REGENERATION OF THE SCIATIC-NERVE IN RATS - THE EFFECT OF MUSCLE BASEMENT-MEMBRANE
    GLASBY, MA
    GSCHMEISSNER, S
    HITCHCOCK, RJI
    HUANG, CLH
    [J]. JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 1986, 68 (05): : 829 - 833