Polysaccharide Films Built by Simultaneous or Alternate Spray: A Rapid Way to Engineer Biomaterial Surfaces

被引:36
作者
Cado, G. [1 ]
Kerdjoudj, H. [2 ]
Chassepot, A. [3 ,4 ]
Lefort, M. [1 ]
Benmlih, K. [3 ,4 ]
Hemmerle, J. [3 ,4 ]
Voegel, J. -C. [3 ,4 ]
Jierry, L. [1 ,5 ,6 ]
Schaaf, P. [1 ,5 ,6 ]
Frere, Y. [1 ]
Boulmedais, F. [1 ,6 ]
机构
[1] Ctr Natl Rech Sci, Inst Charles Sadron, UPR 22, F-67034 Strasbourg, France
[2] Univ Reims, UFR Odontol, EA 4691, SFR Cap Sante, F-51095 Reims, France
[3] INSERM, UMR 977, F-67085 Strasbourg, France
[4] Univ Strasbourg, Fac Chirurg Dent, F-67000 Strasbourg, France
[5] Univ Strasbourg, Ecole Europeenne Chim Polymeres & Mat, F-67087 Strasbourg, France
[6] Int Ctr Frontier Res Chem, F-67083 Strasbourg, France
关键词
LAYER-BY-LAYER; POLYELECTROLYTE MULTILAYER FILMS; ADHESION PROPERTIES; HYALURONIC-ACID; IONIC-STRENGTH; CELL-ADHESION; CROSS-LINKING; CHITOSAN; HEPARIN; COATINGS;
D O I
10.1021/la300563s
中图分类号
O6 [化学];
学科分类号
070301 [无机化学];
摘要
We investigated polysaccharide films obtained by simultaneous and alternate spraying of a chitosan (CHI) solution as polycation and hyaluronic acid (HA), alginate (ALG), and chondroitin sulfate (CS) solutions as polyanions. For simultaneous spraying, the film thickness increases linearly with the cumulative spraying time and passes through a maximum for polyanion/CHI molar charge ratios lying between 0.6 and 1.2. The size of polyanion/CHI complexes formed in solution was compared with the simultaneously sprayed film growth rate as a function of the polyanion/CHI molar charge ratio. A good correlation was found. This suggests the importance of polyanion/polycation complexation in the simultaneous spraying process. Depending on the system, the film topography is either liquid-like or granular. Film biocompatibility was evaluated using human gingival fibroblasts. A small or no difference is observed in cell viability and adhesion between the two deposition processes. The CHI/HA system appears to be the best for cell adhesion inducing the clustering of CD44, a cell surface HA receptor, at the membrane of cells. Simultaneous or alternate spraying of CHI/HA appears thus to be a convenient and fast procedure for biomaterial surface modifications.
引用
收藏
页码:8470 / 8478
页数:9
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