Chemically Detachable Polyelectrolyte Multilayer Platform for Cell Sheet Engineering

被引：24

作者：

Chassepot, Armelle ^{[1
,2
]}

Gao, Longcheng ^{[3
]}

Nguyen, Isabelle ^{[1
,2
]}

Dochter, Alexandre ^{[6
]}

Fioretti, Florence ^{[1
,2
]}

Menu, Patrick ^{[4
]}

Kerdjoudj, Halima ^{[5
]}

Baehr, Corinne ^{[3
]}

Schaaf, Pierre ^{[6
]}

Voegel, Jean-Claude ^{[1
,2
]}

Boulmedais, Fouzia ^{[6
]}

Frisch, Benoit ^{[3
]}

Ogiert, Joelle ^{[1
,2
]}

机构：

[1] Fac Chirurg Dent, INSERM, U977, F-67085 Strasbourg, France

[2] Univ Strasbourg, Fac Chirurg Dent, F-67000 Strasbourg, France

[3] Univ Strasbourg, CNRS, Lab Concept & Applicat Mol Bioact, Fac Pharm,UMR 7199, F-67401 Illkirch Graffenstaden, France

[4] Fac Med, UHP Nancy 1, Grp Bioengn, UMR CNRS 7561, F-54500 Vandoeuvre Les Nancy, France

[5] UFR Odontol, IFR URCA 53, UMR S 926, INSERM, F-51095 Reims, France

[6] Univ Strasbourg, Inst Charles Sadron, Ctr Natl Rech Sci, UPR 22, F-67034 Strasbourg, France

来源：

CHEMISTRY OF MATERIALS | 2012年 / 24卷 / 05期

关键词：

layer-by-layer; disulfide bond; sacrificial platform; human gingival fibroblasts; autologous cell sheet; contractile phenotype; HUMAN UMBILICAL ARTERIES; VESSEL REPLACEMENT; ENDOTHELIAL-CELLS; PROGENITOR CELLS; GENE DELIVERY; FILMS; DIFFERENTIATION; DETACHMENT; MONOLAYERS; CARRIERS;

D O I：

10.1021/cm2024982

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070305 [高分子化学与物理];

摘要：

Human gingival fibroblasts (HGFs) cell sheets have a potential use for in vivo wound healing due to the ability of HGFs to adopt a contractile phenotype which is typically expressed during extracellular matrix tissue remodeling. For this purpose, we developed a chemically detachable platform based on poly(allylamine hydrochloride)/poly(styrene sulfonate) multilayer film built on a sacrificial precursor film which served as a substrate for HGF cell layer formation. The sacrificial precursor film, based on disulfide-containing polycation and polyanion, is degradable under mild conditions compatible for cell sheet detachment. Cellular viability and cell phenotype analysis of HGF show that the designed platform promotes cell phenotype switch into contractile phenotype, maintained after cell sheet lift-off. This contractile phenotype is acquired by fibroblasts during in vivo wound healing and tissue remodeling. HGFs cell sheet fragments, obtained by this detachment process, could be cultured later on showing a good retention of the typical spindle-shape of differentiated cells after 10 days of culture. HGFs cell sheets have great potential applications as autologous substrates for tissue repair and cellular synthetic platforms for research on connective tissue diseases or evaluation of novel therapeutic agents.

引用

页码：930 / 937

页数：8

共 43 条

[1]

Polyelectrolyte films boost progenitor cell differentiation into endothelium-like monolayers [J].

Berthelemy, Nicolas ;

Kerdjoudj, Halima ;

Gaucher, Caroline ;

Schaaf, Pierre ;

Stolz, Jean-Francois ;

Lacolley, Patrick ;

Voegel, Jean-Claude ;

Menu, Patrick .

ADVANCED MATERIALS, 2008, 20 (14) :2674-+

[2]

O2 Level Controls Hematopoietic Circulating Progenitor Cells Differentiation into Endothelial or Smooth Muscle Cells [J].

Berthelemy, Nicolas ;

Kerdjoudj, Halima ;

Schaaf, Pierre ;

Prin-Mathieu, Christine ;

Lacolley, Patrick ;

Stoltz, Jean-Francois ;

Voegel, Jean-Claude ;

Menu, Patrick .

PLOS ONE, 2009, 4 (05)

[3]

Clinical biocompatibility of biodegradable orthopaedic implants for internal fixation:: a review [J].

Böstman, O ;

Pihlajamäki, H .

BIOMATERIALS, 2000, 21 (24) :2615-2621

[4]

Endothelial cell - interactions with polyelectrolyte multilayer films [J].

Boura, C ;

Muller, S ;

Vautier, D ;

Dumas, D ;

Schaaf, P ;

Voegel, JC ;

Stoltz, JF ;

Menu, P .

BIOMATERIALS, 2005, 26 (22) :4568-4575

[5]

Endothelial cells grown on thin polyelectrolyte mutlilayered films: an evaluation of a new versatile surface modification [J].

Boura, C ;

Menu, P ;

Payan, E ;

Picart, C ;

Voegel, JC ;

Muller, S ;

Stoltz, JF .

BIOMATERIALS, 2003, 24 (20) :3521-3530

[6]

Free-Standing and Reactive Thin Films Fabricated by Covalent Layer-by-Layer Assembly and Subsequent Lift-Off of Azlactone-Containing Polymer Multilayers [J].

Buck, Maren E. ;

Lynn, David M. .

LANGMUIR, 2010, 26 (20) :16134-16140

[7]

Ultrathin multilayer polyelectrolyte films on gold: Construction and thickness determination .1. [J].

Caruso, F ;

Niikura, K ;

Furlong, DN ;

Okahata, Y .

LANGMUIR, 1997, 13 (13) :3422-3426

[8]

A cleavable-polycation template method for the fabrication of noncrosslinked, porous polyelectrolyte multilayered films [J].

Chen, Jun ;

Xia, Xi-Ming ;

Huang, Shi-Wen ;

Zhuo, Ren-Xi .

ADVANCED MATERIALS, 2007, 19 (07) :979-+

[9]

Development of cell-selective films for layered co-culturing of vascular progenitor cells [J].

Chong, Mark S. K. ;

Chan, Jerry ;

Choolani, Mahesh ;

Lee, Chuen-Neng ;

Teoh, Swee-Hin .

BIOMATERIALS, 2009, 30 (12) :2241-2251

[10]

Fuzzy nanoassemblies: Toward layered polymeric multicomposites [J].

Decher, G .

SCIENCE, 1997, 277 (5330) :1232-1237

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