Preparation and catalytic performance of lipases encapsulated in sol-gel materials

被引:13
作者
Kato, K [1 ]
Gong, YF [1 ]
Saito, T [1 ]
Yokogawa, Y [1 ]
机构
[1] Natl Inst AIST, Ceram Res Inst, Biofunct Ceram Grp, Moriyama Ku, Nagoya, Aichi 4638560, Japan
关键词
lipase; enantioselectivity; sol-gel; encapsulated; vinyltriethoxysilane;
D O I
10.1271/bbb.66.221
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three kinds of lipases (from Candida antarctica, Pseudomonas cepacia, and Pseudomonas fluorescens) were encapsulated in inorganic matrices by the sol-gel method in order to synthesize chiral compounds by kinetic resolution. Sol-gel lipases Prepared with vinyltriethoxysilane had higher hydrolysis activity for 2-octyl acetate than those with other silane precursors: tetramethoxysilane, methyltrimethoxysilane, and propyltrimethoxysilane.
引用
收藏
页码:221 / 223
页数:3
相关论文
共 13 条
[1]  
BANJIC JD, 2001, ORG LETT, V13, P2025
[2]   Aqueous sol-gel process for protein encapsulation [J].
Bhatia, RB ;
Brinker, CJ ;
Gupta, AK ;
Singh, AK .
CHEMISTRY OF MATERIALS, 2000, 12 (08) :2434-2441
[3]  
Carrea G, 2000, ANGEW CHEM INT EDIT, V39, P2226
[4]   QUANTITATIVE-ANALYSES OF BIOCHEMICAL KINETIC RESOLUTIONS OF ENANTIOMERS [J].
CHEN, CS ;
FUJIMOTO, Y ;
GIRDAUKAS, G ;
SIH, CJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (25) :7294-7299
[5]   Patterned hexagonal arrays of living cells in sol-gel silica films [J].
Chia, SY ;
Urano, J ;
Tamanoi, F ;
Dunn, B ;
Zink, JI .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (27) :6488-6489
[6]  
Faber K., 2000, Biotransformations in Organic Chemistry, V4th
[7]   Encapsulation of biologicals within silicate, siloxane, and hybrid sol-gel polymers: An efficient and generic approach [J].
Gill, I ;
Ballesteros, A .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (34) :8587-8598
[8]   Efficient preparation of optically active ketoprofen by Mucor java']javanicus lipase immobilized on an inorganic support [J].
Kato, K ;
Gong, YF ;
Saito, T ;
Kimoto, H .
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2000, 90 (03) :332-334
[9]   Sol-gel encapsulated anti-trinitrotoluene antibodies in immunoassays for TNT [J].
Lan, EH ;
Dunn, B ;
Zink, JI .
CHEMISTRY OF MATERIALS, 2000, 12 (07) :1874-1878
[10]  
Reetz MT, 1996, BIOTECHNOL BIOENG, V49, P527, DOI 10.1002/(SICI)1097-0290(19960305)49:5<527::AID-BIT5>3.0.CO