Efficient preparation of optically active ketoprofen by Mucor java']javanicus lipase immobilized on an inorganic support

被引：31

作者：

Kato, K ^{[1
]}

Gong, YF ^{[1
]}

Saito, T ^{[1
]}

Kimoto, H ^{[1
]}

机构：

[1] Natl Ind Res Inst Nagoya, Dept Chem, Kita Ku, Nagoya, Aichi 4628510, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2000年 / 90卷 / 03期

关键词：

ketoprofen; resolution; enantioselective; lipase; Mucor [!text type='java']java[!/text]nicus;

D O I：

10.1263/jbb.90.332

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipase M from Mucor javanicus, one of nine commercially available hydrolytic enzymes, showed good enantioselectivity (E=50) for racemic ketoprofen trifluoroethyl ester in phosphate buffer (pH 7.0) containing 30% acetone. Lipase M immobilized on Toyonite 200-A showed the best selectivity (E=55) and reactivity. Moreover, the lipase could be recycled at least 5 times.

引用

页码：332 / 334

页数：3

共 10 条

[1] QUANTITATIVE-ANALYSES OF BIOCHEMICAL KINETIC RESOLUTIONS OF ENANTIOMERS [J].

CHEN, CS ;

FUJIMOTO, Y ;

GIRDAUKAS, G ;

SIH, CJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (25) :7294-7299

[2] A FACILE ENZYMATIC RESOLUTION PROCESS FOR THE PREPARATION OF (+)-S-2-(6-METHOXY-2-NAPHTHYL)PROPIONIC ACID (NAPROXEN) [J].

GU, QM ;

CHEN, CS ;

SIH, CJ .

TETRAHEDRON LETTERS, 1986, 27 (16) :1763-1766

[3]

HAMON DPG, 1995, TETRAHEDRON, V51, P12645

[4]

HEUVEL M, 1997, TETRAHEDRON LETT, V38, P1655

[5] Immobilization of lipase on a new inorganic ceramics support, toyonite, and the reactivity and enantioselectivity of the immobilized lipase [J].

Kamori, M ;

Hori, T ;

Yamashita, Y ;

Hirose, Y ;

Naoshima, Y .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2000, 9 (4-6) :269-274

[6] Optical resolution of 2-(3-indolyl)propionic acid with Mucor java']javanicus and α-chymotrypsin [J].

Kato, K ;

Gong, Y ;

Tanaka, S ;

Katayama, M ;

Kimoto, H .

BIOTECHNOLOGY LETTERS, 1999, 21 (05) :457-461

[7] Preparation of optically active trifluoromethylated (3′-indolyl) thiacarboxylic acids, novel plant growth regulators, through lipase-catalyzed enantioselective hydrolysis [J].

Kato, K ;

Tanaka, S ;

Fujii, S ;

Katayama, M ;

Kimoto, H .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 87 (01) :76-81

[8]

LBELMONTE MT, 1997, J ORG CHEM, V62, P1831

[9]

Park HJ, 1999, J BIOSCI BIOENG, V87, P545

[10]

Schmid RD, 1998, ANGEW CHEM INT EDIT, V37, P1609

← 1 →