Evaluation of Vitamin D Standardization Program protocols for standardizing serum 25-hydroxyvitamin D data: a case study of the program's potential for national nutrition and health surveys

被引：140

作者：

Cashman, Kevin D. ^{[1
,2
]}

Kiely, Mairead ^{[1
]}

Kinsella, Michael ^{[1
]}

Durazo-Arvizu, Ramon A. ^{[3
]}

Tian, Lu ^{[4
]}

Zhang, Yue ^{[1
]}

Lucey, Alice ^{[1
]}

Flynn, Albert ^{[1
]}

Gibney, Michael J. ^{[5
]}

Vesper, Hubert W. ^{[6
]}

Phinney, Karen W. ^{[7
]}

Coates, Paul M. ^{[8
]}

Picciano, Mary F. ^{[8
]}

Sempos, Christopher T. ^{[8
]}

机构：

[1] Natl Univ Ireland Univ Coll Cork, Sch Food & Nutr Sci, Cork, Ireland

[2] Natl Univ Ireland Univ Coll Cork, Dept Med, Cork, Ireland

[3] Loyola Univ, Stritch Sch Med, Dept Prevent Med & Epidemiol, Chicago, IL 60611 USA

[4] Stanford Univ, Sch Med, Stanford, CA 94305 USA

[5] Univ Coll Dublin, Inst Food & Hlth, Dublin 2, Ireland

[6] CDC, Natl Ctr Environm Hlth, Atlanta, GA 30333 USA

[7] NIST, Div Analyt Chem, Gaithersburg, MD 20899 USA

[8] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA

来源：

AMERICAN JOURNAL OF CLINICAL NUTRITION | 2013年 / 97卷 / 06期

关键词：

CIRCULATING; 25-HYDROXYVITAMIN-D; ASSAYS; ACCURATE; ADULTS; D-3;

D O I：

10.3945/ajcn.112.057182

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Background: The Vitamin D Standardization Program (VDSP) has developed protocols for standardizing procedures of 25-hydroxyvitamin D [25(OH)D] measurement in National Health/Nutrition Surveys to promote 25(OH)D measurements that are accurate and comparable over time, location, and laboratory procedure to improve public health practice. Objective: We applied VDSP protocols to existing ELISA-derived serum 25(OH)D data from the Irish National Adult Nutrition Survey (NANS) as a case-study survey and evaluated their effectiveness by comparison of the protocol-projected estimates with those from a reanalysis of survey serums by using liquid chromatography-tandem mass spectrometry (LC-tandem MS). Design: The VDSP reference system and protocols were applied to ELISA-based serum 25(OH)D data from the representative NANS sample (n = 1118). A reanalysis of 99 stored serums by using standardized LC-tandem MS and resulting regression equations yielded predicted standardized serum 25(OH)D values, which were then compared with LC-tandem MS reanalyzed values for all serums. Results: Year-round prevalence rates for serum 25(OH)D concentrations <30, <40, and <50 nmol/L were 6.5%, 21.9%, and 40.0%, respectively, via original ELISA measurements and 11.4%, 25.3%, and 43.7%, respectively, when VDSP protocols were applied. Differences in estimates at <30- and <40-nmol/L thresholds, but not at the <50-nmol/L threshold, were significant (P < 0.05). A reanalysis of all serums by using LC-tandem MS confirmed prevalence estimates as 11.2%, 27.2%, and 45.0%, respectively. Prevalences of serum 25(OH)D concentrations >125 nmol/L were 1.2%, 0.3%, and 0.6% by means of ELISA, VDSP protocols, and LC-tandem MS, respectively. Conclusion: VDSP protocols hold a major potential for national nutrition and health surveys in terms of the standardization of serum 25(OH)D data.

引用

页码：1235 / 1242

页数：8

共 32 条

[21]

National Committee for Clinical Laboratory Standards (NCCLS), 1999, C37A NCCLS CLIN LAB

[22]

Ruston D., 2004, The National Diet Nutrition Survey: adults aged 19 to 64 years. Nutritional status (anthropometry and blood analytes), V4

[23]

Sempos C. T., 1992, Australian Journal of Nutrition and Dietetics, V49, P96

[24] Vitamin D status as an international issue: National surveys and the problem of standardization [J].

Sempos, Christopher T. ;

Vesper, Hubert W. ;

Phinney, Karen W. ;

Thienpont, Linda M. ;

Coates, Paul M. .

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2012, 72 :32-40

[25] C-3 epimers can account for a significant proportion of total circulating 25-hydroxyvitamin D in infants, complicating accurate measurement and interpretation of vitamin D status [J].

Singh, Ravinder J. ;

Taylor, Robert L. ;

Reddy, G. Satyanarayana ;

Grebe, Stefan K. G. .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2006, 91 (08) :3055-3061

[26] Candidate Reference Measurement Procedures for Serum 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 by Using Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry [J].

Stepman, Hedwig C. M. ;

Vanderroost, An ;

Van Uytfanghe, Katleen ;

Thienpont, Linda M. .

CLINICAL CHEMISTRY, 2011, 57 (03) :441-448

[27] Specifications for trueness and precision of a reference measurement system for serum/plasma 25-hydroxyvitamin D analysis [J].

Stoeckl, Dietmar ;

Sluss, Patrick M. ;

Thienpont, Linda M. .

CLINICA CHIMICA ACTA, 2009, 408 (1-2) :8-13

[28] Development of a Candidate Reference Measurement Procedure for the Determination of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 in Human Serum Using Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry [J].

Tai, Susan S. -C. ;

Bedner, Mary ;

Phinney, Karen W. .

ANALYTICAL CHEMISTRY, 2010, 82 (05) :1942-1948

[29] Standardization of measurements of 25-Hydroxyvitamin D3 and D2 [J].

Thienpont, Linda M. ;

Stepman, Hedwig C. M. ;

Vesper, Hubert W. .

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 2012, 72 :41-49

[30]

Tian L, STAT MED

← 1 2 3 4 →