Proteome analysis identifies L1CAM/CD171 and DPP4/CD26 as novel markers of human skin mast cells

被引：30

作者：

Gschwandtner, M. ^{[1
]}

Paulitschke, V. ^{[2
]}

Mildner, M. ^{[1
]}

Brunner, P. M. ^{[2
]}

Hacker, S. ^{[3
]}

Eisenwort, G. ^{[4
,5
]}

Sperr, W. R. ^{[4
,5
]}

Valent, P. ^{[4
,5
]}

Gerner, C. ^{[6
,7
]}

Tschachler, E. ^{[1
]}

机构：

[1] Med Univ Vienna, Res Div Biol & Pathobiol Skin, Vienna, Austria

[2] Med Univ Vienna, Dept Dermatol, Vienna, Austria

[3] Med Univ Vienna, Dept Plast & Reconstruct Surg, Vienna, Austria

[4] Med Univ Vienna, Dept Internal Med 1, Div Hematol & Hemostaseol, Vienna, Austria

[5] Med Univ Vienna, Ludwig Boltzmann Cluster Oncol, Vienna, Austria

[6] Univ Vienna, Fac Chem, Inst Analyt Chem, Vienna, Austria

[7] Med Univ Vienna, Ctr Comprehens Canc, Inst Canc Res, Vienna, Austria

来源：

ALLERGY | 2017年 / 72卷 / 01期

基金：

奥地利科学基金会;

关键词：

bone marrow; mastocytosis; proteomics; psoriasis; skin homeostasis; MEMBRANE ANTIGEN PHENOTYPE; ADHESION MOLECULE L1; LARGE GENE LISTS; FC-EPSILON-RI; PEPTIDASE-IV; ENDOTHELIAL-CELLS; DISEASES; MASTOCYTOSIS; BASOPHILS; DIFFERENTIATION;

D O I：

10.1111/all.12919

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

BackgroundThe function of skin mast cells has been well documented in IgE-mediated allergic reactions, whereas other mast cell functions are poorly defined. This study aimed at identifying novel mast cell proteins by proteome analysis of primary human skin mast cells. MethodsThe proteome of skin mast cells was compared to other cell types and analyzed using bioinformatics. The expression and function of two proteins hitherto not described in skin mast cells was investigated in isolated mast cells as well as in mast cells in situ. ResultsWithin the mast cell proteome, we identified 49 highly expressed proteins previously not described in mast cells; 21 of these proteins were found to be selectively expressed in mast cells. Two proteins, the neural cell adhesion molecule L1 and dipeptidyl peptidase 4, were further studied. L1 was found to be highly expressed in mast cells in normal, psoriasis, and mastocytosis skin. Dipeptidyl peptidase 4 was found to be expressed in mast cells in normal, psoriasis, and mastocytosis skin as well as in bone marrow mast cells in patients with systemic mastocytosis. In normal skin, mast cells were identified as a major source of dipeptidyl peptidase 4 and we also found that skin mast cells and fibroblasts secrete an active form of this enzyme. ConclusionsIn a systematic proteomics approach we identified two novel mast cell proteins potentially relevant to skin homeostasis: neural cell adhesion molecule L1 and dipeptidyl peptidase 4.

引用

页码：85 / 97

页数：13

共 52 条

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