Phase-fluorometry study on dielectric relaxation of acrylodan-labeled human serum albumin

被引：7

作者：

Buzády, A

Erostyák, J

Somogyi, B

机构：

[1] Univ Pecs, Inst Phys, Dept Expt Phys, H-7624 Pecs, Hungary

[2] Univ Pecs, Dept Biophys, H-7625 Pecs, Hungary

[3] Univ Pecs, Jobin Yvon Mol Luminescence Reference Lab, H-7624 Pecs, Hungary

[4] Off Acad Res Grp Attached Univ & Other Inst, Res Grp Fluorescence Spect, H-7625 Pecs, Hungary

来源：

BIOPHYSICAL CHEMISTRY | 2001年 / 94卷 / 1-2期

关键词：

dielectric relaxation; acrylodan; human serum albumin; phase-fluorometry; time-emission matrix;

D O I：

10.1016/S0301-4622(01)00212-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dielectric relaxation (DR) of acrylodan-labeled human serum albumin (HSA/AC) was studied by phase-fluorometry. A non-monoexponential behavior of both the total fluorescence - and the DR decays has been found. The protein environment of the fluorescent marker shows DR times ranging from the pico to nanosecond timescale. In fluorescence emission decays measured on the red side of the fluorescence spectrum a time constant (< 10 ps) affected by a negative preexponential was found supporting the existence of DR of the excited states. (C) 2001 Elsevier Science B.V. All rights reserved.

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页码：75 / 85

页数：11

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