Large-scale gene trapping in C57BL/6N mouse embryonic stem cells

被引：99

作者：

Hansen, Gwenn M. ^{[1
]}

Markesich, Diane C. ^{[1
]}

Burnett, Michael B. ^{[1
]}

Zhu, Qichao ^{[1
]}

Dionne, Karen M. ^{[1
]}

Richter, Lizabeth J. ^{[1
]}

Finnell, Richard H. ^{[2
]}

Sands, Arthur T. ^{[1
]}

Zambrowicz, Brian P. ^{[1
]}

Abuin, Alejandro ^{[1
]}

机构：

[1] Lexicon Pharmaceut Inc, The Woodlands, TX 77381 USA

[2] Texas A&M Inst Genom Med, Houston, TX 77030 USA

来源：

GENOME RESEARCH | 2008年 / 18卷 / 10期

关键词：

D O I：

10.1101/gr.078352.108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the construction and analysis of a mouse gene trap mutant resource created in the C57BL/6N genetic background containing more than 350,000 sequence-tagged embryonic stem (ES) cell clones. We also demonstrate the ability of these ES cell clones to contribute to the germline and produce knockout mice. Each mutant clone is identified by a genomic sequence tag representing the exact insertion location, allowing accurate prediction of mutagenicity and enabling direct genotyping of mutant alleles. Mutations have been identified in more than 10,000 genes and show a bias toward the first intron. The trapped ES cell lines, which can be requested from the Texas A&M Institute for Genomic Medicine, are readily available to the scientific community.

引用

页码：1670 / 1679

页数：10

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