The knockout mouse project

被引：446

作者：

Austin, CP

Battey, JF

Bradley, A

Bucan, M

Capecchi, M

Collins, FS

Dove, WF

Duyk, G

Dymecki, S

Eppig, JT

Grieder, FB

Heintz, N

Hicks, G

Insel, TR

Joyner, A

Koller, BH

Lloyd, KCK

Magnuson, T

Moore, MW

Nagy, A

Pollock, JD

Roses, AD

Sands, AT

Seed, B

Skarnes, WC

Snoddy, J

Soriano, P

Stewart, DJ

Stewart, F

Stillman, B

Varmus, H

Varticovski, L

Verma, IM

Vogt, TF

von Melchner, H

Witkowski, J

Woychik, RP

Wurst, W

Yancopoulos, GD

Young, SG

Zambrowicz, B

机构：

[1] NHGRI, NIH, Bethesda, MD 20892 USA

[2] Natl Inst Deafness & Other Commun Disorders, NIH, Bethesda, MD 20892 USA

[3] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[4] Univ Penn, Dept Genet, Philadelphia, PA 19104 USA

[5] Univ Utah, Eccles Inst Human Genet, Salt Lake City, UT 85112 USA

[6] Univ Wisconsin, McArdle Lab Canc Res, Madison, WI 53706 USA

[7] TPG Ventures, San Francisco, CA 94104 USA

[8] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

[9] Jackson Lab, Bar Harbor, ME 04609 USA

[10] NIH, Natl Ctr Res Resources, Bethesda, MD 20817 USA

[11] Rockefeller Univ, Mol Biol Lab, New York, NY 10021 USA

[12] Manitoba Inst Cell Biol, Winnipeg, MB R3E 0V9, Canada

[13] NIMH, Bethesda, MD 20892 USA

[14] Skirball Inst Biomol Med, New York, NY 10016 USA

[15] Univ N Carolina, Dept Genet, Chapel Hill, NC 27599 USA

[16] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA

[17] Deltagen, Redwood City, CA 94063 USA

[18] Univ Toronto, Samuel Lumenfeld Res Inst, Toronto, ON M5G 1X5, Canada

[19] Natl Inst Drug Abuse, Bethesda, MD 20892 USA

[20] GlaxoSmithKline Inc, Res Triangle Pk, NC 27709 USA

[21] Lexicon Genet, The Woodlands, TX 77381 USA

[22] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA

[23] Oak Ridge Natl Lab, Univ Tennessee ORNL Grad Sch Genome Sci & Technol, Oak Ridge, TN 37831 USA

[24] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

[25] Cold Spring Harbor Lab, Banbury Ctr, Cold Spring Harbor, NY 11724 USA

[26] Univ Technol, Bioz, MPI CBG, D-1307 Dresden, Germany

[27] Mem Sloan Kettering Canc Ctr, New York, NY 10021 USA

[28] NCI, NIH, Bethesda, MD 20892 USA

[29] Salk Inst Biol Studies, Mol Biol & Virol Lab, La Jolla, CA 92037 USA

[30] Merck Res Labs, West Point, PA 19486 USA

[31] Goethe Univ Frankfurt, Sch Med, Lab Mol Hematol, D-60590 Frankfurt, Germany

[32] Jackson Lab, Bar Harbor, ME 04609 USA

[33] Max Planck Inst Psychiat, GSF Res Ctr, Inst Dev Genet, D-85764 Munich, Germany

[34] Regeneron Pharmaceut Inc, Tarrytown, NY 10591 USA

[35] Univ Calif San Francisco, Gladstone Fdn Cardiovasc Dis, San Francisco, CA 94143 USA

来源：

NATURE GENETICS | 2004年 / 36卷 / 09期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/ng0904-921

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

Mouse knockout technology provides a powerful means of elucidating gene function in vivo, and a publicly available genome-wide collection of mouse knockouts would be significantly enabling for biomedical discovery. To date, published knockouts exist for only about 10% of mouse genes. Furthermore, many of these are limited in utility because they have not been made or phenotyped in standardized ways, and many are not freely available to researchers. It is time to harness new technologies and efficiencies of production to mount a high-throughput international effort to produce and phenotype knockouts for all mouse genes, and place these resources into the public domain.

引用

页码：921 / 924

页数：4

共 25 条

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