Hydrolysis of microcystins and nodularin by microwave radiation

被引：18

作者：

Reichelt, M ^{[1
]}

Hummert, C ^{[1
]}

Luckas, B ^{[1
]}

机构：

[1] Univ Jena, Inst Nutr & Environm, D-07743 Jena, Germany

来源：

CHROMATOGRAPHIA | 1999年 / 49卷 / 11-12期

关键词：

column liquid chromatography; microwave hydrolysis; microcystin; nodularin; D- and L-selective amino acid determination;

D O I：

10.1007/BF02466910

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110 degrees C. Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160 degrees C results in complete cleavage of peptide bonds and high recoveries of amino acids. Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization with ortho-phthaldialdehyde and the chiral thiol N-iso-butyryl-cysteine.

引用

页码：671 / 677

页数：7

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