Transgenic tobacco and peas expressing a proteinase inhibitor from Nicotiana alata have increased insect resistance

被引:41
作者
Charity, JA
Anderson, MA
Bittisnich, DJ
Whitecross, M
Higgins, TJV
机构
[1] CSIRO, Div Plant Ind, Canberra, ACT 2601, Australia
[2] La Trobe Univ, Sch Biochem, Bundoora, Vic 3083, Australia
[3] Australian Natl Univ, CRC Plant Sci, Canberra, ACT 2601, Australia
[4] Australian Natl Univ, Dept Bot & Zool, Canberra, ACT 2601, Australia
关键词
Helicoverpa armigera; insect resistance; proteinase inhibitor; protein processing; transgenic tobacco and pea;
D O I
10.1023/A:1009633710224
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Proteinase inhibitors have been used to increase resistance to insect pests in transgenic plants. A cDNA clone encoding a multi-domain proteinase inhibitor precursor from Nicotiana alata (Na-PI) was transferred into tobacco and peas under the control of a promoter from a ribulose-1, 5-bisphosphate carboxylase small subunit gene. The Na-PI precursor was cleaved in the leaves of transgenic tobacco and peas, and M-r 6000 polypeptides accumulated to levels of 0.3% and 0.1%, respectively, of the total soluble protein. The Na-PI cDNA segregated as a dominant Mendelian trait and was stably transmitted for at least two generations of both species. Helicoverpa armigera larvae that ingested tobacco or pea leaves containing Na-PI exhibited higher mortality or were delayed in growth and development relative to control larvae.
引用
收藏
页码:357 / 365
页数:9
相关论文
共 42 条
[1]  
[Anonymous], [No title captured]
[2]   PROTEINASE-INHIBITORS IN NICOTIANA-ALATA STIGMAS ARE DERIVED FROM A PRECURSOR PROTEIN WHICH IS PROCESSED INTO 5 HOMOLOGOUS INHIBITORS [J].
ATKINSON, AH ;
HEATH, RL ;
SIMPSON, RJ ;
CLARKE, AE ;
ANDERSON, MA .
PLANT CELL, 1993, 5 (02) :203-213
[3]   COLORADO POTATO BEETLES (LEPTINOTARSA-DECEMLINEATA) ADAPT TO PROTEINASE-INHIBITORS INDUCED IN POTATO LEAVES BY METHYL JASMONATE [J].
BOLTER, CJ ;
JONGSMA, MA .
JOURNAL OF INSECT PHYSIOLOGY, 1995, 41 (12) :1071-1078
[4]   ADDITIVE PROTECTIVE EFFECTS OF DIFFERENT PLANT-DERIVED INSECT RESISTANCE GENES IN TRANSGENIC TOBACCO PLANTS [J].
BOULTER, D ;
EDWARDS, GA ;
GATEHOUSE, AMR ;
GATEHOUSE, JA ;
HILDER, VA .
CROP PROTECTION, 1990, 9 (05) :351-354
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]  
Broadway RM, 1996, ARCH INSECT BIOCHEM, V32, P39, DOI 10.1002/(SICI)1520-6327(1996)32:1&lt
[7]  
39::AID-ARCH3&gt
[8]  
3.0.CO
[9]  
2-S
[10]   THE EFFECT OF DIETARY-PROTEIN ON THE GROWTH AND DIGESTIVE PHYSIOLOGY OF LARVAL HELIOTHIS-ZEA AND SPODOPTERA-EXIGUA [J].
BROADWAY, RM ;
DUFFEY, SS .
JOURNAL OF INSECT PHYSIOLOGY, 1986, 32 (08) :673-680