Distribution of the multidrug efflux pump genes, adeABC, adeDE and adeIJK, and class 1 integron genes in multiple-antimicrobial-resistant clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex

被引:91
作者
Lin, Li [1 ,2 ]
Ling, Bao-Dong [1 ,2 ]
Li, Xian-Zhi [3 ]
机构
[1] N Sichuan Med Coll, Inst Mat Med, Nanchong 637007, Sichuan, Peoples R China
[2] N Sichuan Med Coll, Dept Pharmacol, Nanchong 637007, Sichuan, Peoples R China
[3] Hlth Canada, Hlth Prod & Food Branch, Vet Drugs Directorate, Human Safety Div, Ottawa, ON K1A 0K9, Canada
关键词
Acinetobacter; Efflux pump genes; Integron; Multidrug resistance; STRAINS; EPIDEMIOLOGY; SYSTEM;
D O I
10.1016/j.ijantimicag.2008.06.027
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Of 112 non-repetitive clinical isolates of Acinetobacter baumannii-Acinetobacter calcoaceticus complex, 80% were resistant to a variety of structurally unrelated antimicrobials although all isolates were susceptible to minocycline and polymyxin. Resistance to carbapenems occurred in 8% of the isolates. The presence of adeSR-adeABC, adeDE and adeIJK drug efflux system genes and class 1 integron genes (integrase gene int1) was assessed by polymerase chain reaction (PCR) in relation to the susceptibility of the isolates to 20 antimicrobials. The majority of isolates (75%) with high levels of multidrug resistance were positive for adeSR-adeABC and adeIJK as well as int1 and thus belong to A. baumannii (i.e. genomospecies 2). Positive adeE was only observed in adeSR-adeABC/adeIJK/int1-negative isolates (8%; likely belonging to Acinetobacter genomospecies 3) that were relatively susceptible to several agents, and adeE expression was undetectable. The results reveal a possible association between adeABC/adeIJK and int1 in multidrug-resistant isolates of A. baumannii. In addition, differential distribution of the resistance-nodulation-cell division (RND) genes can likely be used as indicators for differentiating Acinetobacter species. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.
引用
收藏
页码:27 / 32
页数:6
相关论文
共 15 条
[1]  
[Anonymous], 2012, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard
[2]   Acinetobacter spp, as nosocomial pathogens: Microbiological, clinical, and epidemiological features [J].
BergogneBerezin, E ;
Towner, KJ .
CLINICAL MICROBIOLOGY REVIEWS, 1996, 9 (02) :148-+
[3]   Novel resistance-nodulation-cell division efflux system AdeDE in Acinetobacter genomic DNA group 3 [J].
Chau, SL ;
Chu, YW ;
Houang, ETS .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (10) :4054-4055
[4]   Presence of active efflux systems AdeABC, AdeDE and AdeXYZ in different Acinetobacter genomic DNA groups [J].
Chu, YW ;
Chau, SL ;
Houang, ETS .
JOURNAL OF MEDICAL MICROBIOLOGY, 2006, 55 (04) :477-478
[5]   AdeIJK, a resistance-nodulation-cell division pump effluxing multiple antibiotics in Acinetobacter baumannii [J].
Damier-Piolle, Laurence ;
Magnet, Sophie ;
Bremont, Sylvie ;
Lambert, Thierry ;
Courvalin, Patrice .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (02) :557-562
[6]   Comparative genomics of multidrug resistance in Acinetobacter baumannii [J].
Fournier, Pierre-Edouard ;
Vallenet, David ;
Barbe, Valerie ;
Audic, Stephane ;
Ogata, Hiroyuki ;
Poirel, Laurent ;
Richet, Herve ;
Robert, Catherine ;
Mangenot, Sophie ;
Abergel, Chantal ;
Nordmann, Patrice ;
Weissenbach, Jean ;
Raoult, Didier ;
Claverie, Jean-Michel .
PLOS GENETICS, 2006, 2 (01) :62-72
[7]   Epidemiology of rifampin ADP-ribosyltransferase (arr-2) and metallo-β-lactamase (blaIMP-4) gene cassettes in class 1 integrons in Acinetobacter strains isolated from blood cultures in 1997 to 2000 [J].
Houang, ETS ;
Chu, YW ;
Lo, WS ;
Chu, KY ;
Cheng, AFB .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (04) :1382-1390
[8]   Carbapenemase and efflux pump genes in Acinetobacter calcoaceticus-Acinetobacter baumannii complex strains from Singapore [J].
Koh, Tse Hsien ;
Sng, Li-Hwei ;
Wang, Grace Chee Yeng ;
Hsu, Li-Yang ;
Zhao, Yi .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2007, 60 (05) :1173-1174
[9]   Efflux-mediated drug resistance in bacteria [J].
Li, XZ ;
Nikaido, H .
DRUGS, 2004, 64 (02) :159-204
[10]   Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454 [J].
Magnet, S ;
Courvalin, P ;
Lambert, T .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (12) :3375-3380