Adducts of oxylipin electrophiles to glutathione reflect a 13 specificity of the downstream lipoxygenase pathway in the tobacco hypersensitive response

被引:74
作者
Davoine, C
Falletti, O
Douki, T
Iacazio, G
Ennar, N
Montillet, JL
Triantaphylidès, C
机构
[1] CEA, DSV, Dept Ecophysiol Vegetale & Microbiol, Lab Radiobiol Vegetale, F-13108 St Paul Les Durance, France
[2] CEA, Dept Rech Fondamentale Mat Condensee, Unite Mixte Rech E3, CEA,UJF,DSM,Serv Chim Inorgan & Biol,Lab Les Acid, F-38054 Grenoble 9, France
[3] Fac Sci & Tech St Jerome, CNRS, UMR 6517, Case 432,Lab Bioinorgan Struct, F-13397 Marseille 20, France
关键词
D O I
10.1104/pp.105.074690
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The response to reactive electrophile species (RES) is now considered as part of the plant response to pathogen and insect attacks. Thanks to a previously established high-performance liquid chromatography tandem mass spectrometry methodology, we have investigated the production of oxylipin RES adducts to glutathione (GSH) during the hypersensitive response (HR) of plants. We have observed that RES conjugation to GSH in tobacco (Nicotiana tabacum) leaves is facile and nonspecific. In cryptogein-elicited tobacco leaves, we show that the oxylipin RES adducts to GSH are produced in correlation with GSH consumption, increase in glutathione S- transferase activity, and the appearance of the cell death symptoms. In this model, the adducts arise mainly from the downstream 13 lipoxygenase (LOX) metabolism, although the induced 9 LOX pathway leads massively to the accumulation of upstream metabolites. The main adducts were obtained from 2-hexenal and 12-oxophytodienoic acid. They accumulate transiently as 1-hexanol-3-GSH, a reduced adduct, and 12-oxo-phytodienoicacid-GSH, respectively. RES conjugation does not initiate cell death but explains part of the GSH depletion that accompanies HR cell death. The nature of these GSH conjugates shows the key role played by the 13 LOX pathway in RES signaling in the tobacco HR.
引用
收藏
页码:1484 / 1493
页数:10
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[11]   Conjugation of keto fatty acids to glutathione in plant tissues.: Characterization and quantification by HPLC-tandem mass spectrometry [J].
Davoine, C ;
Douki, T ;
Iacazio, G ;
Montillet, JL ;
Triantaphylidès, C .
ANALYTICAL CHEMISTRY, 2005, 77 (22) :7366-7372
[12]   Protection Against Cancer by Plant Phenylpropenoids: Induction of Mammalian Anticarcinogenic Enzymes [J].
Dinkova-Kostova, A. T. .
MINI-REVIEWS IN MEDICINAL CHEMISTRY, 2002, 2 (06) :595-610
[13]   Plant glutathione transferases [J].
Dixon, David P. ;
Lapthorn, Adrian ;
Edwards, Robert .
GENOME BIOLOGY, 2002, 3 (03)
[14]   Jasmonates and related oxylipins in plant responses to pathogenesis and herbivory [J].
Farmer, EE ;
Alméras, E ;
Krishnamurthy, V .
CURRENT OPINION IN PLANT BIOLOGY, 2003, 6 (04) :372-378
[15]   The lipoxygenase pathway [J].
Feussner, I ;
Wasternack, C .
ANNUAL REVIEW OF PLANT BIOLOGY, 2002, 53 :275-297
[16]   Local and systemic responses of antioxidants to tobacco mosaic virus infection and to salicylic acid in tobacco - Role in systemic acquired resistance [J].
Fodor, J ;
Gullner, G ;
Adam, AL ;
Barna, B ;
Komives, T ;
Kiraly, Z .
PLANT PHYSIOLOGY, 1997, 114 (04) :1443-1451
[17]   Lipid peroxidation during the hypersensitive response in potato in the absence of 9-lipoxygenases [J].
Göbel, C ;
Feussner, I ;
Rosahl, S .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (52) :52834-52840
[18]   The role and regulation of programmed cell death in plant-pathogen interactions [J].
Greenberg, JT ;
Yao, N .
CELLULAR MICROBIOLOGY, 2004, 6 (03) :201-211
[19]  
HABIG WH, 1974, J BIOL CHEM, V242, P5329
[20]   Silencing of hydroperoxide lyase and allene oxide synthase reveals substrate and defense signaling crosstalk in Nicotiana attenuata [J].
Halitschke, R ;
Ziegler, J ;
Keinänen, M ;
Baldwin, IT .
PLANT JOURNAL, 2004, 40 (01) :35-46