Preliminary application of liquid chromatography-electrospray-ionization mass spectrometry to the detection of 5-methyltetrahydrofolic acid monoglutamate in human plasma

被引:25
作者
Nelson, BC [1 ]
Dalluge, JJ [1 ]
Margolis, SA [1 ]
机构
[1] Natl Inst Stand & Technol, Div Analyt Chem, Adv Chem Sci Lab, Gaithersburg, MD 20899 USA
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2001年 / 765卷 / 02期
关键词
folates; methyltetrahydrofolic acid;
D O I
10.1016/S0378-4347(01)00412-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Liquid chromatography (LC) in direct combination with mass spectrometry (MS) has been shown to be a good analytical technique for the selective separation and detection of labile folate monoglutamates. Reversed-phase LC and electrospray-ionization MS conditions were developed and optimized for the separation and detection of 5-methyltetrahydrofolic acid, 5-formyl tetrahydrofolic acid, tetrahydrofolic acid, dihydrofolic acid and folic acid in aqueous samples. Representative and reproducible positive ion mass spectra were generated for each folate under mild MS conditions. The selective MS detection and identification of endogenous 5-methyltetrahydrofolic acid in human plasma was accomplished through the development of a straightforward C-18-based solid-phase extraction procedure. This procedure allows for the qualitative assessment of 5-methyltetrahydrofolic acid in plasma. Based upon an isotope-dilution internal standard calibration study with standards, the LC-MS limit of quantitation for 5M-THF was estimated to be 0.39 ng/ml. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:141 / 150
页数:10
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