Identification of defensins in human lymphocyte nuclei

被引:29
作者
Blomqvist, M
Bergquist, J
Westman, A
Håkansson, K
Håkansson, P
Fredman, P
Ekman, R [1 ]
机构
[1] Sahlgrenska Univ Hosp Molndal, Dept Psychiat & Neurochem, Inst Clin Neurosci, SE-43180 Molndal, Sweden
[2] Div Ion Phys, Angstrom Lab, Uppsala, Sweden
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 263卷 / 02期
关键词
defensin; lymphocyte; matrix assisted laser desorption/ionization time of flight mass spectrometry; electrospray ionisation time of flight mass spectrometry; electrospray ionisation fourier transform ion cyclotron resonance mass spectrometry;
D O I
10.1046/j.1432-1327.1999.00495.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cell nucleus plays an essential role in all aspects of cell function and regulation. Most of the nuclear proteins/ peptides are synthesized in the cytoplasm. and transported into the nucleus through the nuclear pore complexes. The nuclear proteins/peptides conjugate with each other and interact in transcriptional activation/inactivation. Several of the high molecular mass transcription factors (> 30 kDa) have been identified and-characterized. However, the information on the low molecular mass proteins/peptides of the nucleus is limited. We have investigated these low molecular mass proteins/peptides from the nucleus of human peripheral blood lymphocytes using reversed-phase high-performance liquid chromatography (RP-HPLC). The HPLC fractions were further analysed by matrix assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, electrospray ionization time of flight (ESI-TOF) mass spectrometry and: electrospray ionization fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry for mass determination. Using this combination of mass spectrometry techniques and microsequence analysis, we have shown that human, lymphocyte nuclei contain defensins, a mixture of human neutrophil granule peptide 1, 2 and 3.
引用
收藏
页码:312 / 318
页数:7
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