Genome-wide analysis of mRNAs targeted to yeast mitochondria

被引:239
作者
Marc, P [1 ]
Margeot, A [1 ]
Devaux, F [1 ]
Blugeon, C [1 ]
Corral-Debrinski, M [1 ]
Jacq, C [1 ]
机构
[1] Ecole Normale Super, CNRS, UMR 8541, Mol Genet Lab, F-75005 Paris, France
关键词
D O I
10.1093/embo-reports/kvf025
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is agreed that nuclear-encoded mitochondrial proteins are post-translationally targeted to mitochondria, even if, in some cases, a co-translational phase can assist the import of precursor proteins. We used yeast DNA microarrays to analyse the mRNA populations associated with free and mitochondrion-bound polysomes. As expected, many mRNAs, known to encode mitochondrial proteins, are localized to free cytoplasmic polysomes, but many are localized to mitochondrion-bound polysomes. Furthermore, the 3'-UTR of six randomly chosen mitochondrion-bound mRNAs contains sufficient information to target, in vivo, non-translatable RNA to the vicinity of mitochondria. Interestingly, genes producing mRNAs that are targeted to mitochondria are mainly of ancient bacterial origin, whereas those producing mRNAs that are translated in the cytoplasm are mainly of eukaryotic origin. These observations, which support the recent hypotheses concerning the dual origin of the mitochondrial proteome, provide new insights into the biogenesis of mitochondria.
引用
收藏
页码:159 / 164
页数:6
相关论文
共 33 条
[31]   Candida tropicalis Etr1p and Saccharomyces cerevisiae Ybr026p (Mrf1′p), 2-enoyl thioester reductases essential for mitochondrial respiratory competence [J].
Torkko, JM ;
Koivuranta, KT ;
Miinalainen, IJ ;
Yagi, AI ;
Schmitz, W ;
Kastaniotis, AJ ;
Airenne, TT ;
Gurvitz, A ;
Hiltunen, KJ .
MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (18) :6243-6253
[32]   CO-TRANSLATIONAL PROTEIN IMPORT INTO MITOCHONDRIA - AN ALTERNATIVE VIEW [J].
VERNER, K .
TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (10) :366-371
[33]   A PROTEIN COMPLEX REQUIRED FOR SIGNAL-SEQUENCE-SPECIFIC SORTING AND TRANSLOCATION [J].
WIEDMANN, B ;
SAKAI, H ;
DAVIS, TA ;
WIEDMANN, M .
NATURE, 1994, 370 (6489) :434-440