Single-cell sequencing-based technologies will revolutionize whole-organism science

被引:842
作者
Shapiro, Ehud [1 ,2 ]
Biezuner, Tamir [1 ,2 ]
Linnarsson, Sten [3 ]
机构
[1] Weizmann Inst Sci, Dept Comp Sci & Appl Math, IL-76100 Rehovot, Israel
[2] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel
[3] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
基金
瑞典研究理事会; 欧洲研究理事会;
关键词
DNA METHYLATION PATTERNS; MESSENGER-RNA-SEQ; GENE-EXPRESSION; GENOME AMPLIFICATION; TRANSCRIPTIONAL LANDSCAPE; CDNA AMPLIFICATION; TUMOR-CELLS; EVOLUTION; REVEALS; LINEAGE;
D O I
10.1038/nrg3542
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The unabated progress in next-generation sequencing technologies is fostering a wave of new genomics, epigenomics, transcriptomics and proteomics technologies. These sequencing-based technologies are increasingly being targeted to individual cells, which will allow many new and longstanding questions to be addressed. For example, single-cell genomics will help to uncover cell lineage relationships; single-cell transcriptomics will supplant the coarse notion of marker-based cell types; and single-cell epigenomics and proteomics will allow the functional states of individual cells to be analysed. These technologies will become integrated within a decade or so, enabling high-throughput, multi-dimensional analyses of individual cells that will produce detailed knowledge of the cell lineage trees of higher organisms, including humans. Such studies will have important implications for both basic biological research and medicine.
引用
收藏
页码:618 / 630
页数:13
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