Single-cell sequencing-based technologies will revolutionize whole-organism science

被引：842

作者：

Shapiro, Ehud ^{[1
,2
]}

Biezuner, Tamir ^{[1
,2
]}

Linnarsson, Sten ^{[3
]}

机构：

[1] Weizmann Inst Sci, Dept Comp Sci & Appl Math, IL-76100 Rehovot, Israel

[2] Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel

[3] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden

来源：

NATURE REVIEWS GENETICS | 2013年 / 14卷 / 09期

基金：

瑞典研究理事会; 欧洲研究理事会;

关键词：

DNA METHYLATION PATTERNS; MESSENGER-RNA-SEQ; GENE-EXPRESSION; GENOME AMPLIFICATION; TRANSCRIPTIONAL LANDSCAPE; CDNA AMPLIFICATION; TUMOR-CELLS; EVOLUTION; REVEALS; LINEAGE;

D O I：

10.1038/nrg3542

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The unabated progress in next-generation sequencing technologies is fostering a wave of new genomics, epigenomics, transcriptomics and proteomics technologies. These sequencing-based technologies are increasingly being targeted to individual cells, which will allow many new and longstanding questions to be addressed. For example, single-cell genomics will help to uncover cell lineage relationships; single-cell transcriptomics will supplant the coarse notion of marker-based cell types; and single-cell epigenomics and proteomics will allow the functional states of individual cells to be analysed. These technologies will become integrated within a decade or so, enabling high-throughput, multi-dimensional analyses of individual cells that will produce detailed knowledge of the cell lineage trees of higher organisms, including humans. Such studies will have important implications for both basic biological research and medicine.

引用

页码：618 / 630

页数：13

共 144 条

[81] Automated analysis of embryonic gene expression with cellular resolution in C-elegans [J].

Murray, John Isaac ;

Bao, Zhirong ;

Boyle, Thomas J. ;

Boeck, Max E. ;

Mericle, Barbara L. ;

Nicholas, Thomas J. ;

Zhao, Zhongying ;

Sandel, Matthew J. ;

Waterston, Robert H. .

NATURE METHODS, 2008, 5 (08) :703-709

[82] Multidimensional regulation of gene expression in the C. elegans embryo [J].

Murray, John Isaac ;

Boyle, Thomas J. ;

Preston, Elicia ;

Vafeados, Dionne ;

Mericle, Barbara ;

Weisdepp, Peter ;

Zhao, Zhongying ;

Bao, Zhirong ;

Boeck, Max ;

Waterston, Robert H. .

GENOME RESEARCH, 2012, 22 (07) :1282-1294

[83] The transcriptional landscape of the yeast genome defined by RNA sequencing [J].

Nagalakshmi, Ugrappa ;

Wang, Zhong ;

Waern, Karl ;

Shou, Chong ;

Raha, Debasish ;

Gerstein, Mark ;

Snyder, Michael .

SCIENCE, 2008, 320 (5881) :1344-1349

[84] Tumour evolution inferred by single-cell sequencing [J].

Navin, Nicholas ;

Kendall, Jude ;

Troge, Jennifer ;

Andrews, Peter ;

Rodgers, Linda ;

McIndoo, Jeanne ;

Cook, Kerry ;

Stepansky, Asya ;

Levy, Dan ;

Esposito, Diane ;

Muthuswamy, Lakshmi ;

Krasnitz, Alex ;

McCombie, W. Richard ;

Hicks, James ;

Wigler, Michael .

NATURE, 2011, 472 (7341) :90-U119

[85] Targeted capture and massively parallel sequencing of 12 human exomes [J].

Ng, Sarah B. ;

Turner, Emily H. ;

Robertson, Peggy D. ;

Flygare, Steven D. ;

Bigham, Abigail W. ;

Lee, Choli ;

Shaffer, Tristan ;

Wong, Michelle ;

Bhattacharjee, Arindam ;

Eichler, Evan E. ;

Bamshad, Michael ;

Nickerson, Deborah A. ;

Shendure, Jay .

NATURE, 2009, 461 (7261) :272-U153

[86] The stem cell population of the human colon crypt:: Analysis via methylation patterns [J].

Nicolas, Pierre ;

Kim, Kyoung-Mee ;

Shibata, Darryl ;

Tavare, Simon .

PLOS COMPUTATIONAL BIOLOGY, 2007, 3 (03) :364-374

[87] Detecting antigens by quantitative immuno-PCR [J].

Niemeyer, Christof M. ;

Adler, Michael ;

Wacker, Ron .

NATURE PROTOCOLS, 2007, 2 (08) :1918-1930

[88] The Life History of 21 Breast Cancers [J].

Nik-Zainal, Serena ;

Van Loo, Peter ;

Wedge, David C. ;

Alexandrov, Ludmil B. ;

Greenman, Christopher D. ;

Lau, King Wai ;

Raine, Keiran ;

Jones, David ;

Marshall, John ;

Ramakrishna, Manasa ;

Shlien, Adam ;

Cooke, Susanna L. ;

Hinton, Jonathan ;

Menzies, Andrew ;

Stebbings, Lucy A. ;

Leroy, Catherine ;

Jia, Mingming ;

Rance, Richard ;

Mudie, Laura J. ;

Gamble, Stephen J. ;

Stephens, Philip J. ;

McLaren, Stuart ;

Tarpey, Patrick S. ;

Papaemmanuil, Elli ;

Davies, Helen R. ;

Varela, Ignacio ;

McBride, David J. ;

Bignell, Graham R. ;

Leung, Kenric ;

Butler, Adam P. ;

Teague, Jon W. ;

Martin, Sancha ;

Joensson, Goran ;

Mariani, Odette ;

Boyault, Sandrine ;

Miron, Penelope ;

Fatima, Aquila ;

Langerod, Anita ;

Aparicio, Samuel A. J. R. ;

Tutt, Andrew ;

Sieuwerts, Anieta M. ;

Borg, Ake ;

Thomas, Gilles ;

Salomon, Anne Vincent ;

Richardson, Andrea L. ;

Borresen-Dale, Anne-Lise ;

Futreal, P. Andrew ;

Stratton, Michael R. ;

Campbell, Peter J. .

CELL, 2012, 149 (05) :994-1007

[89] Mutational Processes Molding the Genomes of 21 Breast Cancers [J].

Nik-Zainal, Serena ;

Alexandrov, Ludmil B. ;

Wedge, David C. ;

Van Loo, Peter ;

Greenman, Christopher D. ;

Raine, Keiran ;

Jones, David ;

Hinton, Jonathan ;

Marshall, John ;

Stebbings, Lucy A. ;

Menzies, Andrew ;

Martin, Sancha ;

Leung, Kenric ;

Chen, Lina ;

Leroy, Catherine ;

Ramakrishna, Manasa ;

Rance, Richard ;

Lau, King Wai ;

Mudie, Laura J. ;

Varela, Ignacio ;

McBride, David J. ;

Bignell, Graham R. ;

Cooke, Susanna L. ;

Shlien, Adam ;

Gamble, John ;

Whitmore, Ian ;

Maddison, Mark ;

Tarpey, Patrick S. ;

Davies, Helen R. ;

Papaemmanuil, Elli ;

Stephens, Philip J. ;

McLaren, Stuart ;

Butler, Adam P. ;

Teague, Jon W. ;

Jonsson, Goran ;

Garber, Judy E. ;

Silver, Daniel ;

Miron, Penelope ;

Fatima, Aquila ;

Boyault, Sandrine ;

Langerod, Anita ;

Tutt, Andrew ;

Martens, John W. M. ;

Aparicio, Samuel A. J. R. ;

Borg, Ake ;

Salomon, Anne Vincent ;

Thomas, Gilles ;

Borresen-Dale, Anne-Lise ;

Richardson, Andrea L. ;

Neuberger, Michael S. .

CELL, 2012, 149 (05) :979-993

[90] Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases [J].

Noctor, SC ;

Martínez-Cerdeño, V ;

Ivic, L ;

Kriegstein, AR .

NATURE NEUROSCIENCE, 2004, 7 (02) :136-144

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