Simple method for monitoring enzymatic reactions by direct injection of the medium reaction in a gas chromatograph equipped with packed liner

被引：3

作者：

daSilva, FM

Bastos, FD

Oestreicher, EG

Pinto, GF

Jones, J

Paiva, LMC

机构：

[1] UNIV FED RIO DE JANEIRO,INST QUIM,DEPT BIOQUIM,BR-21945900 RIO JANEIRO,BRAZIL

[2] UNIV FED RIO DE JANEIRO,CTR TECNOL,INST QUIM,DEPT QUIM ORGAN,BR-21945900 RIO JANEIRO,BRAZIL

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 10期

关键词：

D O I：

10.1023/A:1018444301693

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reduction of sulcatone, catalyzed by Thermoanaerobium brockii alcohol dehydrogenase in Tris/HCl buffer (pH 7.8) using 2-propanol 20% (v/v) as an NADPH regeneration system could be followed by using a gas chromatograph equipped with a packed liner that allowed the direct injection of the aqueous medium reaction. The time-course curve obtained by the analytical method here described and that obtained by spectrophotometric methods were similar.

引用

收藏

页码：739 / 741

页数：3

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