Osmotic destruction of Saccharomyces cerevisiae is not related to a high water flow rate across the membrane

被引：8

作者：

Beney, L ^{[1
]}

de Marañón, IM ^{[1
]}

Marechal, PA ^{[1
]}

Moundanga, S ^{[1
]}

Gervais, P ^{[1
]}

机构：

[1] Univ Bourgogne 1, Ecole Natl Super Biol Appl Nutr & Alimentat, Lab Genie Procedes Alimentaires & Biotechnol, F-21000 Dijon, France

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2001年 / 9卷 / 03期

关键词：

kinetic parameters; mass transfer; membranes; viability; water activity; yeast;

D O I：

10.1016/S1369-703X(01)00145-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Influence of the kinetics of osmotic pressure variation on yeast viability was related to the rate of water transfer across the yeast membrane. A very high value of membrane hydraulic permeability (L-p > 6x10(-11) m s(-1) Pa-1) of the yeast Saccharomyces cerevisiae implies the presence of aqueous pores in the yeast membrane and explains the yeast resistance to a very high osmotic flow. The high water flow rate can not explain, by itself, the cell mortality following an osmotic shock. Experiments performed at different osmotic pressure levels show that the yeast death under osmotic shifts was related to the coupled effects of the kinetics of osmotic pressure variation and of the total osmotic pressure level of the medium. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：205 / 210

页数：6

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