Fluctuations as a source of information in fluorescence microscopy

被引：17

作者：

Petrasek, Zdenek ^{[1
]}

Schwille, Petra ^{[1
]}

机构：

[1] Tech Univ Dresden, Biophys Grp, Biotechnol Zentrum, D-01307 Dresden, Germany

来源：

JOURNAL OF THE ROYAL SOCIETY INTERFACE | 2009年 / 6卷

关键词：

fluorescence spectroscopy; imaging; single molecule; IMAGE CORRELATION SPECTROSCOPY; SINGLE-MOLECULE FLUORESCENCE; CROSS-CORRELATION SPECTROSCOPY; RESONANCE ENERGY-TRANSFER; INTENSITY DISTRIBUTION ANALYSIS; PHOTON ARRIVAL TRAJECTORIES; LASER-SCANNING MICROSCOPE; PARTICLE TRACKING; LIVING CELLS; HIGH-ORDER;

D O I：

10.1098/rsif.2008.0200.focus

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fluctuations in fluorescence spectroscopy and microscopy have traditionally been regarded as noise they lower the resolution and contrast and do not permit high acquisition rates. However, fluctuations can also be used to gain additional information about a system. This fact has been exploited in single-point microscopic techniques, such as fluorescence correlation spectroscopy and analysis of single molecule trajectories, and also in the imaging field, e. g. in spatio-temporal image correlation spectroscopy. Here, we discuss how fluctuations are used to obtain more quantitative information from the data than that given by average values, while minimizing the effects of noise due to stochastic photon detection.

引用

页码：S15 / S25

页数：11

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