Molecular cloning of Frizzled-10, a novel member of the Frizzled gene family

被引：168

作者：

Koike, J

Takagi, A

Miwa, T

Hirai, M

Terada, M

Katoh, M

机构：

[1] Natl Canc Ctr, Res Inst, Div Genet, Chuo Ku, Tokyo 1040045, Japan

[2] Tokai Univ, Dept Internal Med, Div Gastroenterol 2, Isehara, Kanagawa 2591193, Japan

[3] Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130033, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 262卷 / 01期

关键词：

WNT receptor; tissue polarity gene; beta-catenin;

D O I：

10.1006/bbrc.1999.1161

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The frizzled genes encode WNT receptors. Frizzled-10 (FZD10), a novel member of the Frizzled gene family, has been cloned and characterized. Nucleotide sequence analysis showed that human FZD10 gene encodes a seven-transmembrane-receptor of 581 amino acids, with the N-terminal cysteine-rich domain and the C-terminal Ser/Thr-Xxx-Val motif. Larger amounts of FZD10 mRNA, 4.0 kb in size, were detected in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, FZD10 mRNA was abundant in the cerebellum. Among cancer cell lines, FZD10 was highly expressed in a cervical cancer cell line, HeLa S3, and moderately in a colon cancer cell line, SW480. The FZD10 gene was mapped to human chromosome 12q24.33. FZD10 shares 65.7% amino-acid identity with Frizzled-9 (FZD9). FZD10 and FZD9 constitute a subfamily among the Frizzled genes. (C) 1999 Academic Press.

引用

页码：39 / 43

页数：5

共 26 条

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