A sunflower leaf antifungal peptide active against Sclerotinia sclerotiorum

被引：9

作者：

Regente, MC

Oliva, CR

Feldman, ML

Castagnaro, AP

delaCanal, L

机构：

[1] UNIV NACL MAR DEL PLATA,FAC CIENCIAS EXACTAS & NAT,INST INVEST BIOL,RA-7600 MAR DEL PLATA,ARGENTINA

[2] UNIV NACL TUCUMAN,INSIBIO,RA-4000 S MIGUEL TUCUMAN,TUCUMAN,ARGENTINA

来源：

PHYSIOLOGIA PLANTARUM | 1997年 / 100卷 / 01期

关键词：

antifungal proteins; Helianthus annuus; Sclerotinia sclerotiorum; sunflower;

D O I：

10.1034/j.1399-3054.1997.1000121.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A 5-kDa antifungal peptide (AP5) was isolated from Helianthus annuus L. (line HA89) leaves infected with a virulent isolate of Sclerotinia sclerotiorum (Lib.) de Bary. AP5 was purified by gel filtration, cation exchange chromatography and reverse phase FPLC and HPLC. This peptide in vitro inhibits ascospores germination of the fungal pathogen S. sclerotiorum or produces mycelial growth inhibition, depending on its concentration. The effective concentration of AP5 giving 50% growth inhibition (IC50) against S. sclerotiorum was 0.4 mu M. The antifungal efficacy of AP5 is higher than that of other antimicrobial proteins already described that have no appreciable effect on S. sclerotiorum below 4 mu M. The relevance of this finding with regard to the function of AP5 in sunflower resistance to pathogens is discussed.

引用

页码：178 / 182

页数：5

共 21 条

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