Handling of picoliter liquid samples in a poly(dimethylsiloxane)-based microfluidic device

被引:215
作者
Hosokawa, K [1 ]
Fujii, T [1 ]
Endo, I [1 ]
机构
[1] RIKEN, Inst Phys & Chem Res, Biochem Syst Lab, Wako, Saitama 3510198, Japan
关键词
D O I
10.1021/ac990571d
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Transportation, metering, and mixing of picoliter-sized liquid samples were realized in a microfluidic device with a main working area of one square millimeter. The device was constructed by sealing microfabricated grooves on a chip made of poly(dimethylsiloxane) (PDMS). Two different samples were segmented into 600-pL droplets in a microchannel with a cross section of W (100 mu m) x H (25 mu m), and the droplets were merged together. For acceleration of the mixing, the merged droplet was shuttled back and forth. Recirculation in a moving droplet was proven to be effective for high-speed mixing in this diffusion-dominated scale. All the handling operations were carried out using air pressure transferred through microfabricated vent valves which have been newly developed. The demonstrated strategy, including fabrication, leads to high-performance and low-cost micro total analysis systems (mu TAS).
引用
收藏
页码:4781 / 4785
页数:5
相关论文
共 25 条
  • [1] ANDERSON RC, 1998, P MICR TOT AN SYST 9, P11
  • [2] ANDERSON RC, 1997, P 1997 INT C SOL STA, P477
  • [3] [Anonymous], P MICR TAS 98 BANFF
  • [4] Fast mixing by lamination.
    Branebjerg, J
    Gravesen, P
    Krog, JP
    Nielsen, CR
    [J]. NINTH ANNUAL INTERNATIONAL WORKSHOP ON MICRO ELECTRO MECHANICAL SYSTEMS, IEEE PROCEEDINGS: AN INVESTIGATION OF MICRO STRUCTURES, SENSORS, ACTUATORS, MACHINES AND SYSTEMS, 1996, : 441 - 446
  • [5] Biotechnology at low Reynolds numbers
    Brody, JP
    Yager, P
    Goldstein, RE
    Austin, RH
    [J]. BIOPHYSICAL JOURNAL, 1996, 71 (06) : 3430 - 3441
  • [6] An integrated nanoliter DNA analysis device
    Burns, MA
    Johnson, BN
    Brahmasandra, SN
    Handique, K
    Webster, JR
    Krishnan, M
    Sammarco, TS
    Man, PM
    Jones, D
    Heldsinger, D
    Mastrangelo, CH
    Burke, DT
    [J]. SCIENCE, 1998, 282 (5388) : 484 - 487
  • [7] Patterned delivery of immunoglobulins to surfaces using microfluidic networks
    Delamarche, E
    Bernard, A
    Schmid, H
    Michel, B
    Biebuyck, H
    [J]. SCIENCE, 1997, 276 (5313) : 779 - 781
  • [8] STEADY FLOW IN REGION OF CLOSED STREAMLINES IN A CYLINDRICAL CAVITY
    DUDA, JL
    VRENTAS, JS
    [J]. JOURNAL OF FLUID MECHANICS, 1971, 45 (JAN30) : 247 - &
  • [9] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane)
    Duffy, DC
    McDonald, JC
    Schueller, OJA
    Whitesides, GM
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (23) : 4974 - 4984
  • [10] Integrated capillary electrophoresis on flexible silicone microdevices: Analysis of DNA restriction fragments and detection of single DNA molecules on microchips
    Effenhauser, CS
    Bruin, GJM
    Paulus, A
    Ehrat, M
    [J]. ANALYTICAL CHEMISTRY, 1997, 69 (17) : 3451 - 3457