Tissue and subcellular localization of oligofurostanosides and their specific degrading β-glucosidase in Dioscorea caucasica Lipsky

被引：11

作者：

Gurielidze, K ^{[1
]}

Gogoberidze, M ^{[1
]}

Dadeshidze, I ^{[1
]}

Vardosanidze, M ^{[1
]}

Djaoshvilo, M ^{[1
]}

Loinkatsi, N ^{[1
]}

机构：

[1] Durmischidze Inst Biochem & Biotechnol, Dept Cell Culture & Biol Act Cpds, GE-0159 Tbilisi, Georgia

来源：

PHYTOCHEMISTRY | 2004年 / 65卷 / 05期

关键词：

Dioscorea caucasica Lipsky; Dioscoreaceae; steroid glycoside; oligofurostanoside-specific beta-glucosidase; aryl-beta-glucosidase; oligofurostanoside; thylakoide; localization;

D O I：

10.1016/j.phytochem.2003.12.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of the histochemical technique using the Ehrlich reagent showed that the oligofurostanosides-the steroid glycosides of the furostan series-are localized in idioblasts (special cells-receptacles) of the epidermal layer of Dioscorea caucasica leaves. The activity of oligofurostanoside-specific beta-glucosidase was localized mainly in the membrane fraction of the thylakoides and was also found in the Dioscorea caucasica leaves. We have suggested, that the differential tissue, the subcellular localization of the oligofurostanosides and their degrading enzymes provide the maintenance of the furostanol structure of steroid glycosides which is necessary for their transport within the leaf and from the leaf to the rhizome. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：555 / 559

页数：5

共 18 条

[1] Antiviral isoflavonoid sulfate and steroidal glycosides from the fruits of Solanum torvum [J].