Rational Design of Digital Assays

被引：11

作者：

Debski, Pawel R. ^{[1
,2
]}

Gewartowski, Kamil ^{[2
]}

Sulima, Magdalena ^{[2
]}

Kaminski, Tomasz S. ^{[1
,2
]}

Garstecki, Piotr ^{[1
,2
]}

机构：

[1] Polish Acad Sci, Inst Phys Chem, PL-01224 Warsaw, Poland

[2] Curios Diagnost Sp Zoo, PL-01224 Warsaw, Poland

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 16期

基金：

欧洲研究理事会;

关键词：

D O I：

10.1021/acs.analchem.5b00942

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Optimum algorithm for digital assays treats chemical compartments as bits of probabilistic information and arranges these bits in a fractional positional system. Maximization of information gain reduces, by orders of magnitude, the number of partitions required to achieve the requested dynamic range and precision of the assay. The method simplifies the execution of digital analytical methods providing for more accessible use of absolute quantization in research and in diagnostics.

引用

页码：8203 / 8209

页数：7

共 18 条

[1] Bar-Or D., 2012, U.S. Patent Application, Patent No. 20120022081
[2] Colstron Jr B.W., 2010, U.S. Patent Application, Patent No. [20100173394 A1, 20100173394]
[3] Microfluidic digital PCR enables rapid prenatal diagnosis of fetal aneuploidy
Fan, H. Christina
Blumenfeld, Yair J.
El-Sayed, Yasser Y.
Chueh, Jane
Quake, Stephen R.
[J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2009, 200 (05) : 543.e1 - 543.e7
[4] Garstecki P., 2012, [No title captured], Patent No. [EP 2012/004792, 2012004792]
[5] Multifunctional Picoliter Droplet Manipulation Platform and Its Application in Single Cell Analysis
Gu, Shu-Qing
Zhang, Yun-Xia
Zhu, Ying
Du, Wen-Bin
Yao, Bo
Fang, Qun
[J]. ANALYTICAL CHEMISTRY, 2011, 83 (19) : 7570 - 7576
[6] Ismagilov R. F., 2010, U.S. Patent Application, Patent No. [2010/028316, 2010028316]
[7] High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets
Kiss, Margaret Macris
Ortoleva-Donnelly, Lori
Beer, N. Reginald
Warner, Jason
Bailey, Christopher G.
Colston, Bill W.
Rothberg, Jonathon M.
Link, Darren R.
Leamon, John H.
[J]. ANALYTICAL CHEMISTRY, 2008, 80 (23) : 8975 - 8981
[8] Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR
Kreutz, Jason E.
Munson, Todd
Huynh, Toan
Shen, Feng
Du, Wenbin
Ismagilov, Rustem F.
[J]. ANALYTICAL CHEMISTRY, 2011, 83 (21) : 8158 - 8168
[9] The numerical interpretation of fermentation-tube results
McCrady, MH
[J]. JOURNAL OF INFECTIOUS DISEASES, 1915, 17 (01) : 183 - 212
[10] Mullis K.B., 1993, Nobel Lectures, V1997

← 1 2 →