NMDA induces apoptosis and necrosis in neuronal cultures. Increased APP immunoreactivity is linked to apoptotic cells

被引：42

作者：

Lesort, M ^{[1
]}

Esclaire, F ^{[1
]}

Yardin, C ^{[1
]}

Hugon, J ^{[1
]}

机构：

[1] FAC MED LIMOGES,CELL BIOL & HISTOL LAB,U CNRS 6104,NEUROBIOL & CELLULAR PATHOL UNIT,F-87025 LIMOGES,FRANCE

来源：

NEUROSCIENCE LETTERS | 1997年 / 221卷 / 2-3期

关键词：

amyloid precursor protein; apoptosis; necrosis; immunoreactivity; rat neuronal cultures; N-methyl-D-aspartate; extracellular deposits;

D O I：

10.1016/S0304-3940(96)13310-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

In rat neuronal cultures exposed for a prolonged period (16 h) to a low concentration of N-methyl-D-aspartate (NMDA; 20 mu M) two different types of neuronal death were observed, necrosis and apoptosis. The immunocytochemical study of necrotic neurons revealed the absence of amyloid precursor protein (APP) labeling in the cytoplasmic region. In contrast, neuronal apoptosis was associated with an increase in cytoplasmic APP immunoreactivity. In addition, the presence of extracellular APP was also detected around some apoptotic neurons, suggesting that apoptosis could contribute to the presence of extracellular APP. (C) 1997 Elsevier Science Ireland Ltd.

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收藏

页码：213 / 216

页数：4

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