The RecQ gene family in plants

被引:61
作者
Hartung, F [1 ]
Puchta, H [1 ]
机构
[1] Univ Karlsruhe, Inst Bot 2, D-76128 Karlsruhe, Germany
关键词
DNA repair; Holliday junction; recombination; RecQ helicases;
D O I
10.1016/j.jplph.2005.10.013
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
RecQ helicases are conserved throughout all. kingdoms of life regarding their overall structure and function. They are 3'-5' DNA helicases resolving different recombinogenic DNA structures. The RecQ helicases are key factors in a number of DNA repair and recombination pathways involved in the maintenance of genome integrity. In eukaryotes the number of RecQ genes and the structure of RecQ proteins vary strongly between organisms. Therefore, they have been named RecQ-tike genes. Knockouts of several RecQ-tike genes cause severe diseases in animals or harmful cellular phenotypes in yeast. Until now the largest number of RecQ-like genes per organism has been found in plants. Arabiclopsis and rice possess seven different RecQ-like genes each. In the almost completely sequenced genome of the moss Physcomitrella patens at least five RecQ-like genes are present. One of the major present and future research aims is to define putative plant-specific functions and to assign their roles in DNA repair and recombination pathways in relation to RecQ genes from other eukaryotes. Regarding their intron positions, the structures of six RecQ-like genes of dicots and monocots are virtually identical indicating a conservation over a time scale of 150 million years. In contrast to other eukaryotes one gene (RecQsim) exists exclusively in plants. It possesses an interrupted helicase domain but nevertheless seems to have maintained the RecQ function. Owing to a recent gene duplication besides the AtRecQl4A gene an additional RecQ-like gene (AtRecQl4B) exists in the Brassicaceae only. Genetic studies indicate that a AtRecQt4A knockout results in sensitivity to mutagens as welt as an hyper-recombination phenotype. Since AtRecQl4B was still present, both genes must have non-redundant roles. Analysis of plant RecQ-tike genes will. not only increase the knowledge on DNA repair and recombination, but also on the evolution and radiation of protein families. (c) 2005 Elsevier GmbH. All rights reserved.
引用
收藏
页码:287 / 296
页数:10
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