Effect of reactive oxygen species overproduction on osteogenesis of porous titanium implant in the present of diabetes mellitus

被引：106

作者：

Feng, Ya-Fei ^{[1
]}

Wang, Lin ^{[1
]}

Zhang, Yang ^{[1
]}

Li, Xiang ^{[2
]}

Ma, Zhen-Sheng ^{[1
]}

Zou, Ji-Wei ^{[1
]}

Lei, Wei ^{[1
]}

Zhang, Zhi-Yong ^{[3
,4
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Orthoped, Xian 710032, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Mech Engn, State Key Lab Mech Syst & Vibrat, Shanghai 200240, Peoples R China

[3] Shanghai Jiao Tong Univ, Dept Plast & Reconstruct Surg, Shanghai Peoples Hosp 9, Shanghai Key Lab Tissue Engn,Sch Med, Shanghai 200011, Peoples R China

[4] Natl Tissue Engn Ctr China, Shanghai 200241, Peoples R China

来源：

BIOMATERIALS | 2013年 / 34卷 / 09期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Diabetes; Reactive oxygen species; Porous titanium; Osteogenesis; Antioxidant; Bone defect; INDUCED OXIDATIVE STRESS; IN-VITRO; ORTHOPEDIC APPLICATIONS; BONE-FORMATION; CELLS; DIFFERENTIATION; COMPLICATIONS; OSTEOPOROSIS; OSTEOBLASTS; DISEASE;

D O I：

10.1016/j.biomaterials.2012.12.023

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Clinical evidence indicates diabetes as a major risk factor for titanium implant treatment with high failure rates and poor osteointegration, but the underlying mechanism involved remains elusive. We hypothesize that reactive oxygen species (ROS) overproduction may contribute to the impaired osteogenesis of porous titanium implants (pTi) under diabetic conditions. To test this hypothesis, we cultured primary rabbit osteoblasts onto pTi and studied the cellular performance when subjected to normal serum (NS), diabetic serum (DS), DS + NAC (a potent ROS inhibitor) and NS + H2O2(an oxidant),In-vivo performance of pTi was investigated by transplanting them intofemoral condyledefects of diabetic rabbits, which received vehicle or NAC treatment respectively. Results showed that diabetic conditions induced significant cellular apoptosis, depressed osteoblast function evidenced by impaired cell attachment and morphology, decreased cell proliferation and differentiation, and compromised in-vivo osteogenesis of pTi, while cellular ROS generation was increased derived from mitochondrial dysfunction. Scavenging ROS with NAC markedly attenuated cell apoptosis and osteoblast dysfunction, and improved bone ingrowth within pTi. Furthermore, treatment with H2O2 exerted similar adverse effect on cellular behavior as diabetes. This study furthers our knowledge on the potential role of ROS overproduction in the diabetes-induced impaired osteogenesis of titanium implants, and indicates anti-oxidative treatment as a promising strategy to promote the treatment efficacy of pTi in diabetic patients. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：2234 / 2243

页数：10