Investigation of Protein Detection Parameters Using Nanofunctionalized Organic Field-Effect Transistors

被引:113
作者
Hammock, Mallory L. [1 ]
Knopfmacher, Oren [1 ]
Naab, Benjamin D. [2 ]
Tok, Jeffrey B. -H. [1 ]
Bao, Zhenan [1 ]
机构
[1] Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA
[2] Stanford Univ, Dept Chem, Stanford, CA 94305 USA
基金
美国国家科学基金会; 瑞士国家科学基金会;
关键词
organic field-effect transistors; biodetection; screening length; nanoparticies; receptor density; DEBYE-SCREENING LENGTH; LABEL-FREE DETECTION; SILICON NANOWIRE; SURFACE COVERAGE; IN-SITU; THROMBIN; SENSORS; GOLD; BIOSENSORS; NANOPARTICLES;
D O I
10.1021/nn305903q
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Biodetection using organic field-effect transistors (OFETs) is gaining increasing interest for applications as diverse as food security, environmental monitoring, and medical diagnostics. However, there still lacks a comprehensive, empirical study on the fundamental limits of OFET sensors. In this paper, we present a thorough study of the various parameters affecting biosensing using an OFET decorated with gold nanoparticle (AuNP) binding sites. These parameters include the spacing between receptors, pH of the buffer, and ionic strength of the buffer. To this end, we employed the thrombin protein and its corresponding DNA binding aptamer to form our model detection system. We demonstrate a detection limit of 100 pM for this protein with high selectivity over other proteases in situ. We describe herein a feasible approach for protein detection with OFETs and a thorough investigation of parameters governing biodetection events using OFETs. Our obtained results should provide important guidelines to tailor the sensors dynamic range to suit other desired OFET-based biodetection applications.
引用
收藏
页码:3970 / 3980
页数:11
相关论文
共 74 条
[1]   Interfacial electronic effects in functional biolayers integrated into organic field-effect transistors [J].
Angione, Maria Daniela ;
Cotrone, Serafina ;
Magliulo, Maria ;
Mallardi, Antonia ;
Altamura, Davide ;
Giannini, Cinzia ;
Cioffi, Nicola ;
Sabbatini, Luigia ;
Fratini, Emiliano ;
Baglioni, Piero ;
Scamarcio, Gaetano ;
Palazzo, Gerardo ;
Torsi, Luisa .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (17) :6429-6434
[2]   Analytical performances of aptamer-based sensing for thrombin detection [J].
Bini, Alessandra ;
Minunni, Maria ;
Tombelli, Sara ;
Centi, Sonia ;
Mascini, Marco .
ANALYTICAL CHEMISTRY, 2007, 79 (07) :3016-3019
[3]   SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].
BOCK, LC ;
GRIFFIN, LC ;
LATHAM, JA ;
VERMAAS, EH ;
TOOLE, JJ .
NATURE, 1992, 355 (6360) :564-566
[4]   Organic field-effect transistor for label-free dopamine sensing [J].
Casalini, Stefano ;
Leonardi, Francesca ;
Cramer, Tobias ;
Biscarini, Fabio .
ORGANIC ELECTRONICS, 2013, 14 (01) :156-163
[5]   Label-free colorimetric detection of picomolar thrombin in blood plasma using a gold nanoparticle-based assay [J].
Chen, Chuan-Kuo ;
Huang, Chih-Ching ;
Chang, Huan-Tsung .
BIOSENSORS & BIOELECTRONICS, 2010, 25 (08) :1922-1927
[6]   Functionalized SnO2 nanobelt field-effect transistor sensors for label-free detection of cardiac troponin [J].
Cheng, Yi ;
Chen, Kan-Sheng ;
Meyer, Nancy L. ;
Yuan, Jing ;
Hirst, Linda S. ;
Chase, P. Bryant ;
Xiong, Peng .
BIOSENSORS & BIOELECTRONICS, 2011, 26 (11) :4538-4544
[7]   Aptamer-Based SERRS Sensor for Thrombin Detection [J].
Cho, Hansang ;
Baker, Brian R. ;
Wachsmann-Hogiu, Sebastian ;
Pagba, Cynthia V. ;
Laurence, Ted A. ;
Lane, Stephen M. ;
Lee, Luke P. ;
Tok, Jeffrey B. -H. .
NANO LETTERS, 2008, 8 (12) :4386-4390
[8]   Double layer capacitance measured by organic field effect transistor operated in water [J].
Cramer, T. ;
Kyndiah, A. ;
Murgia, M. ;
Leonardi, F. ;
Casalini, S. ;
Biscarini, F. .
APPLIED PHYSICS LETTERS, 2012, 100 (14)
[9]   Label-free protein detection based on vertically aligned carbon nanotube gated field-effect transistors [J].
Croce, Robert A., Jr. ;
Vaddiraju, Sagar ;
Chan, Pik-Yiu ;
Seyta, Rea ;
Jain, Faquir C. .
SENSORS AND ACTUATORS B-CHEMICAL, 2011, 160 (01) :154-160
[10]   CRYSTALLIZING PROTEINS - A RATIONAL APPROACH [J].
DARCY, A .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :469-471