Bovine CD18 identified as a species specific receptor for Pasteurella haemolytica leukotoxin

被引：68

作者：

Li, J ^{[1
]}

Clinkenbeard, KD ^{[1
]}

Ritchey, JW ^{[1
]}

机构：

[1] Oklahoma State Univ, Coll Vet Med, Dept Anat Pathol & Pharmacol, Stillwater, OK 74078 USA

来源：

VETERINARY MICROBIOLOGY | 1999年 / 67卷 / 02期

关键词：

Pasteurella haemolytica; leukotoxin; leukocytes; CD18; toxin receptor; ligand blotting;

D O I：

10.1016/S0378-1135(99)00040-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Ligand blotting of Pasteurella haemolytica leukotoxin (LKT) susceptible BL3 bovine lymphoma cell membranes with LKT detected two putative receptors with M-r, of 95 and 100 kDa, whereas no LKT binding to membrane proteins was detected for LKT non-susceptible human leukemic cells. Anti-bovine CD18 and CD11a/CD18 mAb recognized 95 and 100kDa bands from BL3 cell membranes. CD18 isolated from BL3 cell membranes bound LKT. Pre-incubation of BL3 cells with anti-bovine CD18 or CD11a/CD18 mAb caused partial inhibition of LKT-induced leukolysis. Therefore, we propose that bovine CD18 acts as a species-specific leukocyte receptor for P. haemolytica LKT. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：91 / 97

页数：7

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