Bovine CD18 identified as a species specific receptor for Pasteurella haemolytica leukotoxin

被引:68
作者
Li, J [1 ]
Clinkenbeard, KD [1 ]
Ritchey, JW [1 ]
机构
[1] Oklahoma State Univ, Coll Vet Med, Dept Anat Pathol & Pharmacol, Stillwater, OK 74078 USA
关键词
Pasteurella haemolytica; leukotoxin; leukocytes; CD18; toxin receptor; ligand blotting;
D O I
10.1016/S0378-1135(99)00040-1
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ligand blotting of Pasteurella haemolytica leukotoxin (LKT) susceptible BL3 bovine lymphoma cell membranes with LKT detected two putative receptors with M-r, of 95 and 100 kDa, whereas no LKT binding to membrane proteins was detected for LKT non-susceptible human leukemic cells. Anti-bovine CD18 and CD11a/CD18 mAb recognized 95 and 100kDa bands from BL3 cell membranes. CD18 isolated from BL3 cell membranes bound LKT. Pre-incubation of BL3 cells with anti-bovine CD18 or CD11a/CD18 mAb caused partial inhibition of LKT-induced leukolysis. Therefore, we propose that bovine CD18 acts as a species-specific leukocyte receptor for P. haemolytica LKT. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:91 / 97
页数:7
相关论文
共 11 条
[1]   Generation of targeted nonpolar gene insertions and operon fusions in Pasteurella haemolytica and creation of a strain that produces and secretes inactive leukotoxin [J].
Fedorova, ND ;
Highlander, SK .
INFECTION AND IMMUNITY, 1997, 65 (07) :2593-2598
[2]   NONRECIPROCAL COMPLEMENTATION OF THE HLYC AND LKTC GENES OF THE ESCHERICHIA-COLI HEMOLYSIN AND PASTEURELLA-HAEMOLYTICA LEUKOTOXIN DETERMINANTS [J].
FORESTIER, C ;
WELCH, RA .
INFECTION AND IMMUNITY, 1990, 58 (03) :828-832
[3]   RTX toxins recognize a beta 2 integrin on the surface of human target cells [J].
Lally, ET ;
Kieba, IR ;
Sato, A ;
Green, CL ;
Rosenbloom, J ;
Korostoff, J ;
Wang, JF ;
Shenker, BJ ;
Ortlepp, S ;
Robinson, MK ;
Billings, PC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (48) :30463-30469
[4]   HEMOLYTIC-ACTIVITY OF THE PASTEURELLA-HAEMOLYTICA LEUKOTOXIN [J].
MURPHY, GL ;
WHITWORTH, LC ;
CLINKENBEARD, KD ;
CLINKENBEARD, PA .
INFECTION AND IMMUNITY, 1995, 63 (08) :3209-3212
[5]   EXPRESSION CLONING OF A DIPHTHERIA-TOXIN RECEPTOR - IDENTITY WITH A HEPARIN-BINDING EGF-LIKE GROWTH-FACTOR PRECURSOR [J].
NAGLICH, JG ;
METHERALL, JE ;
RUSSELL, DW ;
EIDELS, L .
CELL, 1992, 69 (06) :1051-1061
[6]  
SATO N, 1993, BIOCHEM MOL BIOL INT, V29, P899
[7]   CYTO-TOXIN OF PASTEURELLA-HAEMOLYTICA ACTING ON BOVINE LEUKOCYTES [J].
SHEWEN, PE ;
WILKIE, BN .
INFECTION AND IMMUNITY, 1982, 35 (01) :91-94
[8]  
SUN Y, 1997, P 78 C RES WORK AN D
[9]  
Sun YD, 1998, AM J VET RES, V59, P851
[10]   EXTRACTION AND PARTIAL CHARACTERIZATION OF A LEUKOTOXIN FROM A PLAQUE-DERIVED GRAM-NEGATIVE MICROORGANISM [J].
TSAI, CC ;
MCARTHUR, WP ;
BAEHNI, PC ;
HAMMOND, BF ;
TAICHMAN, NS .
INFECTION AND IMMUNITY, 1979, 25 (01) :427-439