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X-ray crystal structures of 70S ribosome functional complexes

被引：495
作者：
Cate, JH
Yusupov, MM
Yusupova, GZ
Earnest, TN
Noller, HF
机构：
[1] Univ Calif Santa Cruz, Sinsheimer Labs, Ctr Mol Biol RNA, Santa Cruz, CA 95064 USA
[2] Lawrence Berkeley Lab, Phys Biosci Div, Macromol Crystallog Facil, Berkeley, CA 94720 USA
来源：
SCIENCE | 1999年 / 285卷 / 5436期
关键词：
D O I：
10.1126/science.285.5436.2095
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Structures of 70S ribosome complexes containing messenger RNA and transfer RNA (tRNA), or tRNA analogs, have been solved by x-ray crystallography at up to 7.8 angstrom resolution. Many details of the interactions between tRNA and the ribosome, and of the packing arrangements of ribosomal RNA (rRNA) helices in and between the ribosomal subunits, can be seen. Numerous contacts are made between the 30S subunit and the P-tRNA anticodon stem-loop; in contrast, the anticodon region of A-tRNA is much more exposed. A complex network of molecular interactions suggestive of a functional relay is centered around the Long penultimate stem of 16S rRNA at the subunit interface, including interactions involving the "switch" helix and decoding site of 16S rRNA, and RNA bridges from the 50S subunit.
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页码：2095 / 2104
页数：10
相关论文
共 125 条
[21]   Crystal structure of the ribosomal RNA domain essential for binding elongation factors [J].
Correll, CC ;
Munishkin, A ;
Chan, YL ;
Ren, Z ;
Wool, IG ;
Steitz, TA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (23) :13436-13441
[22]  
COWGILL CA, 1984, J BIOL CHEM, V259, P5257
[23]   Identification of an RNA-protein bridge spanning the ribosomal subunit interface [J].
Culver, GM ;
Cate, JH ;
Yusupova, GZ ;
Yusupov, MM ;
Noller, HF .
SCIENCE, 1999, 285 (5436) :2133-2135
[24]  
CULVER GM, UNPUB
[25]   The loop E loop D region of Escherichia coli 5S rRNA:: the solution structure reveals an unusual loop that may be important for binding ribosomal proteins [J].
Dallas, A ;
Moore, PB .
STRUCTURE, 1997, 5 (12) :1639-1653
[26]   Modeling the structure of the ribosome [J].
Easterwood, TR ;
Harvey, SC .
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1995, 73 (11-12) :751-756
[27]  
Fink DL, 1996, RNA, V2, P851
[28]   Paromomycin binding induces a local conformational change in the A-site of 16 S rRNA [J].
Fourmy, D ;
Yoshizawa, S ;
Puglisi, JD .
JOURNAL OF MOLECULAR BIOLOGY, 1998, 277 (02) :333-345
[29]   Structure of the A site of Escherichia coli 16S ribosomal RNA complexed with an aminoglycoside antibiotic [J].
Fourmy, D ;
Recht, MI ;
Blanchard, SC ;
Puglisi, JD .
SCIENCE, 1996, 274 (5291) :1367-1371
[30]   A model of the translational apparatus based on a three-dimensional reconstruction of the Escherichia coli ribosome [J].
Frank, J ;
Verschoor, A ;
Li, YH ;
Zhu, J ;
Lata, RK ;
Radermacher, M ;
Penczek, P ;
Grassucci, R ;
Agrawal, RK ;
Srivastava, S .
BIOCHEMISTRY AND CELL BIOLOGY, 1995, 73 (11-12) :757-765
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