Egg Cell-Secreted EC1 Triggers Sperm Cell Activation During Double Fertilization

被引：240

作者：

Sprunck, Stefanie ^{[1
]}

Rademacher, Svenja ^{[1
]}

Vogler, Frank ^{[1
]}

Gheyselinck, Jacqueline ^{[2
,3
]}

Grossniklaus, Ueli ^{[2
,3
]}

Dresselhaus, Thomas ^{[1
]}

机构：

[1] Univ Regensburg, Biochem Zentrum Regensburg, D-93053 Regensburg, Germany

[2] Univ Zurich, Inst Plant Biol, CH-8008 Zurich, Switzerland

[3] Univ Zurich, Zurich Basel Plant Sci Ctr, CH-8008 Zurich, Switzerland

来源：

SCIENCE | 2012年 / 338卷 / 6110期

基金：

瑞士国家科学基金会;

关键词：

SIGNAL-TRANSDUCTION; ARABIDOPSIS; PLANTS; FUSION;

D O I：

10.1126/science.1223944

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Double fertilization is the defining characteristic of flowering plants. However, the molecular mechanisms regulating the fusion of one sperm with the egg and the second sperm with the central cell are largely unknown. We show that gamete interactions in Arabidopsis depend on small cysteine-rich EC1 (EGG CELL 1) proteins accumulating in storage vesicles of the egg cell. Upon sperm arrival, EC1-containing vesicles are exocytosed. The sperm endomembrane system responds to exogenously applied EC1 peptides by redistributing the potential gamete fusogen HAP2/GCS1 (HAPLESS 2/GENERATIVE CELL SPECIFIC 1) to the cell surface. Furthermore, fertilization studies with ec1 quintuple mutants show that successful male-female gamete interactions are necessary to prevent multiple-sperm cell delivery. Our findings provide evidence that mutual gamete activation, regulated exocytosis, and sperm plasma membrane modifications govern flowering plant gamete interactions.

引用

页码：1093 / 1097

页数：5

共 23 条

[1] Gamete Fusion Is Required to Block Multiple Pollen Tubes from Entering an Arabidopsis Ovule [J].