Sodium channel mRNA in the B104 neuroblastoma cell line

被引：22

作者：

DibHajj, SD

Hinson, AW

Black, JA

Waxman, SG

机构：

[1] YALE UNIV,SCH MED,DEPT NEUROL,NEW HAVEN,CT 06510

[2] VET ADM MED CTR,CTR RES NEUROSCI,EPVA,PVA,W HAVEN,CT 06516

来源：

FEBS LETTERS | 1996年 / 384卷 / 01期

关键词：

in situ hybridization; neuroblastoma; mRNA; RT-PCR; sodium channel;

D O I：

10.1016/0014-5793(96)00273-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

B104 neuroblastoma cells are excitable, but the ion channels underlying electrogenesis in these cells have not been identified, RT-PCR, restriction enzyme analysis and in situ hybridization were used to study sodium channel mRNAs in B104 cells, High levels of sodium channel a-subunit mRNAs III, NaG and Na6 and beta 1-subunit mRNA were detected by RT-PCR in B104 cells, Low levels of types I and II alpha-subunit mRNAs mere also present, In situ hybridization with subtype-specific riboprobes detected sodium channel alpha-subunit mRNAs III, NaG and Na6 and beta 1-subunit mRNA in B104 cells; analysis of the percentage of B104 cells expressing each alpha-subunit mRNA subtype suggests that some cells express the mRNAs for several alpha-subunits.

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收藏

页码：78 / 82

页数：5

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