Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

被引：779

作者：

Gratz, Scott J. ^{[1
]}

Cummings, Alexander M. ^{[2
]}

Nguyen, Jennifer N. ^{[3
]}

Hamm, Danielle C. ^{[4
]}

Donohue, Laura K. ^{[2
]}

Harrison, Melissa M. ^{[4
,6
]}

Wildonger, Jill ^{[3
]}

O'Connor-Giles, Kate M. ^{[1
,2
,5
]}

机构：

[1] Univ Wisconsin, Genet Training Program, Madison, WI 53706 USA

[2] Univ Wisconsin, Genet Lab, Madison, WI 53706 USA

[3] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

[4] Univ Wisconsin, Integrated Program Biochem, Madison, WI 53706 USA

[5] Univ Wisconsin, Lab Cell & Mol Biol, Madison, WI 53706 USA

[6] Univ Wisconsin, Sch Med & Publ Hlth, Dept Biomol Chem, Madison, WI 53706 USA

来源：

GENETICS | 2013年 / 194卷 / 04期

基金：

美国国家卫生研究院;

关键词：

ZINC-FINGER NUCLEASES; INDUCED GAP REPAIR; HOMOLOGOUS RECOMBINATION; GENE; EFFICIENT; MELANOGASTER; MUTAGENESIS; ENDONUCLEASE; INTERFERENCE; REPLACEMENT;

D O I：

10.1534/genetics.113.152710

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

引用

页码：1029 / +

页数：17

共 31 条

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