Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer)

被引:29
作者
van Hooft, WF
Hanotte, O
Wenink, PW
Groen, AF
Sugimoto, Y
Prins, HHT
Teale, A
机构
[1] Agr Univ Wageningen, Dept Environm Sci, Trop Nat Conservat & Vertebrate Ecol Grp, NL-6708 PD Wageningen, Netherlands
[2] Int Livestock Res Inst, Nairobi, Kenya
[3] Agr Univ Wageningen, Dept Anim Sci, Anim Breeding & Genet Grp, NL-6700 AH Wageningen, Netherlands
[4] Shirikawa Inst Anim Genet, Fukushima 961, Japan
[5] Univ Stirling, Inst Aquaculture, Stirling FK9 4LA, Scotland
关键词
African buffalo; bovine; interspecific amplification; microsatellites; polymorphism; Syncerus caffer;
D O I
10.1046/j.1365-2052.1999.00453.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The applicability of bovine autosomal microsatellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel elf seven African buffalo. Amplification was observed for 139 markers (83%), and 101 markers were studied further with 91 (90%) being polymorphic. The mean number of alleles per marker was 5.0 (SE = 0.2) and the mean heterozygosity per marker was 0.61 (SE = 0.03). Considering the overall high level of polymorphism, it was concluded that most bovine microsatellite markers are applicable in African buffalo.
引用
收藏
页码:214 / 220
页数:7
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