Transforming growth factor-β1 upregulates the tight junction and P-glycoprotein of brain microvascular endothelial cells

被引：89

作者：

Dohgu, S

Yamauchi, A

Takata, F

Naito, M

Tsuruo, T

Higuchi, S

Sawada, Y

Kataoka, Y

机构：

[1] Fukuoka Univ, Fac Pharmaceut Sci, Dept Pharmaceut Care & Hlth Sci, Jonan Ku, Fukuoka 8140180, Japan

[2] Kyushu Univ, Dept Medicopharmaceut Sci, Grad Sch Pharmaceut Sci, Higashi Ku, Fukuoka 812, Japan

[3] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo, Japan

来源：

CELLULAR AND MOLECULAR NEUROBIOLOGY | 2004年 / 24卷 / 03期

关键词：

transforming growth factor-beta; blood-brain barrier; permeability; P-glycoprotein; mouse brain endothelial cells;

D O I：

10.1023/B:CEMN.0000022776.47302.ce

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

1. The present study was aimed at elucidating effects of transforming growth factor-beta (TGF-beta) on blood - brain barrier (BBB) functions with mouse brain capillary endothelial (MBEC4) cells. 2. The permeability coefficients of sodium fluorescein and Evans blue albumin for MBEC4 cells and the cellular accumulation of rhodamine 123 in MBEC4 cells were dose-dependently decreased after a 12-h exposure to TGF-beta1 (0.01 - 10 ng/mL). 3. The present study demonstrates that TGF-beta lowers the endothelial permeability and enhances the functional activity of P-gp, suggesting that cellular constituents producing TGF-beta in the brain may keep the BBB functioning.

引用

页码：491 / 497

页数：7

共 19 条

[11] Angiogenesis of the blood-brain barrier in vitro and the function of cerebral pericytes [J].