Rapid monitoring assay of congenital adrenal hyperplasia with microbore high-performance liquid chromatography/electrospray ionization tandem mass spectrometry from dried blood spots

被引：51

作者：

Lai, CC ^{[1
]}

Tsai, CH ^{[1
]}

Tsai, FJ ^{[1
]}

Lee, CC ^{[1
]}

Lin, WD ^{[1
]}

机构：

[1] China Med Coll Hosp, Dept Med Genet & Med Res, Taichung 404, Taiwan

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2001年 / 15卷 / 22期

关键词：

D O I：

10.1002/rcm.493

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

17-Hydroxyprogesterone (17OHP) is the most important plasma parameter for diagnosing and monitoring congenital adrenal hyperplasia (CAH) caused by 21-hydroxylase deficiency. A rapid, simple, and specific method based on microbore high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (mu -HPLC/ESI-MS/MS) was developed to determine the presence of 17OHP on dried filter-paper blood samples from patients with CAH caused by 21-hydroxylase deficiency. 17OHP from dried blood spots formed by the action of Girard reagent P (Girl?) turned out to be a water-soluble hydrazone complex. Derivatization with Girl? led to higher ESI sensitivity for 17OHP. The LC/MS/MS detection of GirP-derivatized 17OHP (GirP-17OHP) was rapid (<3 min). The method is repeatable and reproducible, with CVs <7% and 12%, respectively. This new method was used for direct determination of 17OHP in dried blood specimens obtained from abnormal children and infants of various ages with a detection limit of 10 ng/mL (similar to 12 muL blood). The method described allows for rapid and reliable measurements of 17OHP in dried blood specimens from patients affected by CAH. Copyright (C) 2001 John Wiley & Sons, Ltd.

引用

页码：2145 / 2151

页数：7

共 32 条

[11] Identification of main corticosteroids as illegal feed additives in milk replacers by liquid chromatography atmospheric pressure chemical ionisation mass spectrometry [J].