Specific primers for the detection of vip3A insecticidal gene within a Bacillus thuringiensis collection

被引：24

作者：

Rice, WC ^{[1
]}

机构：

[1] ARS, USDA, RRU, LSU Rice Res Stn, Crowley, LA 70527 USA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 28卷 / 05期

关键词：

D O I：

10.1046/j.1365-2672.1999.00536.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A PCR-based method was developed for the rapid detection of vip3A gene encoding a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. Specific primer combinations (three primers for the normal strand and two primers for the complementary strand) were capable of generating diagnostic fragments that successfully predicted the presence of the gene encoding the Vip3A insecticidal toxin in various B. thuringiensis strains. Specificity of amplicons generated by primer pairs was confirmed by restriction endonuclease digestion and DNA sequence analysis, The evaluation of B, thuringiensis strains for biological activity against insect pests of rice is thus aided by the grouping of strains based on their potential insecticidal spectrum.

引用

页码：378 / 382

页数：5

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